Data from: Temporal identity establishes columnar neuron morphology, connectivity, and function in a Drosophila navigation circuit

Sullivan, Luis F., University of Oregon

Warren, Timothy L., University of Oregon

Doe, Chris Q., University of Oregon

Published Feb 21, 2019 on Dryad. https://doi.org/10.5061/dryad.45177sc

Cite this dataset

Sullivan, Luis F.; Warren, Timothy L.; Doe, Chris Q. (2019). Data from: Temporal identity establishes columnar neuron morphology, connectivity, and function in a Drosophila navigation circuit [Dataset]. Dryad. https://doi.org/10.5061/dryad.45177sc

Abstract

The insect central complex (CX) is a conserved brain region containing 60+ neuronal subtypes, several of which contribute to navigation. It is not known how CX neuronal diversity is generated or how developmental origin of subtypes relates to function. We mapped the developmental origin of four key CX subtypes and found that neurons with similar origin have similar axon/dendrite targeting. Moreover, we found that the temporal transcription factor (TTF) Eyeless/Pax6 regulates the development of two recurrently-connected CX subtypes: Eyeless loss simultaneously produces ectopic P-EN neurons with normal axon/dendrite projections, and reduces the number of E-PG neurons. Furthermore, transient loss of Eyeless during development impairs adult flies' capacity to perform celestial navigation. We conclude that neurons with similar developmental origin have similar connectivity, that Eyeless maintains equal E-PG and P-EN neuron number, and that Eyeless is required for the development of circuits that control adult navigation.

Usage notes

Flight data from ES -Gal4 x UAS-Kir2.1 flies

Data file contains raw flight data collected for fly genotype:Empty Split-Gal4 x UAS-Kir2.1. (Figure7- supplement ). See README for detaails on parsing file

es_uaskirj.csv

ss00096 x Kir data

Data contains flight data collected from ss00096 Split-Gal4 x UAS-Kir2.1 flies. Fig. 7 supplement. See README for details on how to parse.

ss96_uaskirj.csv

ss00096 x UAS-Toy-RNAi

Data from ss00096 Split-Gal4 x UAS Toy-RNAi flies. Figure 8b, Figure 8 Supplement. See README for details on how to parse.

ss96toy.csv

Empty Split x Toy-RNAi

Data from Empty Split-GAl4 x UAS Toy-RNAi flies. Figure 8A, Fig. 8 Supplement. See README for details on how to parse.

estoy.csv

mCherry Heat Shock Control flies

Data from R16B06-Gal4 x ts.Tubulin-Gal80 (10); UAS-mCherryRNAi flies that received heatshock. Data in Fig. 7F and Fig. 7 Supplement. See README for details on how to parse.

mcherry_heatshock.csv

Eyeless-RNAi, no heat pulse

Data from R16B06-Gal4 x ts.Tubulin-Gal80 (10); UAS-EyelessRNAi flies that did not receive heat pulse. Data in Fig. 7E and Fig. 7 Supplement. See README for details on parsing.

eyeless_rnai_control.csv

eyeless_rnai_experiment

Data from R16B06-Gal4 x ts.Tubulin-GAl80 (10); UAS-EyelessRNAi flies that received heat pulse. Data in Fig. 7G/Fig. 7 Supplement. See README for details on how to parse.

README

This README explains formatting of all the data files.