Maternal and cohort effects modulate offspring responses to multiple stressors
Torres, Gabriela et al. (2020), Maternal and cohort effects modulate offspring responses to multiple stressors, Dryad, Dataset, https://doi.org/10.5061/dryad.47d7wm39g
Berried females collected in two seasons (SEASON) were kept in 4 treatments (2 EMBRYONIC SALINITY x 2 EMBRYONIC TEMPERATURE). After embryonic development was completed and larvae hatched, they were distributed in 6 treatments (2 LARVAL SALINITY x 3 LARVAL TEMPERATURE). Survival and developmental time of the first stage was determined.
Survival data (proportion) were first adjusted using the equation p’= [p(n-1)/n+0.5]/n, (n=10 individuals) and then analysed after logistic (= logit) transformation39, following Griffen et al. 2016. For survival, we applied a five-way factorial model containing embryonic salinity (ES), embryonic temperature (ET), larval salinity (LS), larval temperature (LT) and season (S).
The duration of development was analysed using the data corresponding to the larvae reared in seawater because we had high mortality rates at a lower salinity of 20 PSU (see Results). The starting model was reduced to a four-way factorial model (the factor “larval salinity”, LS, was dropped), keeping female (F) as a random factor.
European Commission, Award: 235634