Spatiotemporal variation of the indoor mycobiome in daycare centers

Estensmo, Eva Lena, University of Oslo

Morgado, Luis, University of Oslo

Maurice, Sundy, University of Oslo

Martin-Sanchez, Pedro M., University of Oslo

Engh, Ingeborg B., Mycoteam AS

Mattson, Johan, Mycoteam AS

Kauserud, Håvard, University of Oslo

Skrede, Inger, University of Oslo

eva.estensmo@outlook.com, inger.skrede@ibv.uio.no

Publication date: January 3, 2022

Publisher: Dryad

https://doi.org/10.5061/dryad.4f4qrfj9z

Citation

Estensmo, Eva Lena et al. (2022), Spatiotemporal variation of the indoor mycobiome in daycare centers, Dryad, Dataset, https://doi.org/10.5061/dryad.4f4qrfj9z

Abstract

Abstract Background Children spend considerable time in daycare centers in parts of the world and are exposed to the indoor micro- and mycobiomes of these facilities. The level of exposure to microorganisms varies within and between buildings, depending on occupancy, climate, and season. In order to evaluate indoor air quality, and the effect of usage and seasonality, we investigated the spatiotemporal variation in the indoor mycobiomes of two daycare centers. We collected dust samples from different rooms throughout a year and analyzed their mycobiomes using DNA metabarcoding. Results The fungal community composition in rooms with limited occupancy (auxiliary rooms) was similar to the outdoor samples, and clearly different from the rooms with higher occupancy (main rooms). The main rooms had higher abundance of Ascomycota, while the auxiliary rooms contained comparably more Basidiomycota. We observed a strong seasonal pattern in the mycobiome composition, mainly structured by the outdoor climate. Most markedly, basidiomycetes of the orders Agaricales and Polyporales, mainly reflecting typical outdoor fungi, were more abundant during summer and fall. In contrast, ascomycetes of the orders Saccharomycetales and Capnodiales were dominant during winter and spring. Conclusions Our findings provide clear evidences that the indoor mycobiomes in daycare centers are structured by occupancy as well as outdoor seasonality. We conclude that the temporal variability should be accounted for in indoor mycobiome studies and in the evaluation of indoor air quality of buildings.