Data from: Optimizing mouse surgery with online rectal temperature monitoring and preoperative heat supply. Effects on post-ischemic acute kidney injury.
Marschner, Julian Aurelio, Klinikum der Universität München
Schäfer, Hannah, Klinikum der Universität München
Holderied, Alexander, Klinikum der Universität München
Anders, Hans-Joachim, Klinikum der Universität München
Published Feb 05, 2017 on Dryad.
Cite this dataset
Marschner, Julian Aurelio; Schäfer, Hannah; Holderied, Alexander; Anders, Hans-Joachim (2017). Data from: Optimizing mouse surgery with online rectal temperature monitoring and preoperative heat supply. Effects on post-ischemic acute kidney injury. [Dataset]. Dryad. https://doi.org/10.5061/dryad.4v131
Body temperature affects outcomes of tissue injury. We hypothesized that online body core temperature recording and selective interventions help to standardize peri-interventional temperature control and the reliability of outcomes in experimental renal ischemia reperfusion injury (IRI). We recorded core temperature in up to seven mice in parallel using a Thermes USB recorder and ret-3-iso rectal probes with three different protocols. Setup A: Heating pad during ischemia time; Setup B: Heating pad from incision to wound closure; Setup C: A ventilated heating chamber before surgery and during ischemia time with surgeries performed on a heating pad. Temperature profile recording displayed significant declines upon installing anesthesia. The profile of the baseline experimental setup A revealed that <1 % of the temperature readings were within the target range of 36.5 to 38.5 °C. Setup B and C increased the target range readings to 34.6 ± 28.0 % and 99.3 ± 1.5 %, respectively. Setup C significantly increased S3 tubular necrosis, neutrophil influx, and mRNA expression of kidney injury markers. In addition, using setup C different ischemia times generated a linear correlation with acute tubular necrosis parameters at a low variability, which further correlated with the degree of kidney atrophy 5 weeks after surgery. Changing temperature control setup A to C was equivalent to 10 minutes more ischemia time. We conclude that body temperature drops quickly in mice upon initiating anesthesia. Immediate heat supply, e.g. in a ventilated heating chamber, and online core temperature monitoring can help to standardize and optimize experimental outcomes.
Compilation of ORTR raw data, histopathological and immunopathological scoring, qRT-PCR data for kidney injury markers and kidney weight data. The data refer to the Figures 1, 3, 4 and 7 of the manuscript.