Impairment of endothelial function by aerosol from marijuana leaf vaporizers
Data files
Nov 29, 2023 version files 17.60 GB
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Figure1.zip
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Figure2.zip
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Figure3.zip
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Figure4-1.zip
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Figure4-2.zip
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README.docx
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README.md
Abstract
Methods and Results We exposed 8 rats/group to aerosol generated by two vaporizer systems using marijuana with varying THC levels, in a single pulsatile exposure session of 2 sec/min over 5 min, and measured changes in FMD. To model secondhand exposure, we exposed rats for 1 min to diluted aerosol approximating release of uninhaled Volcano aerosol into typical residential rooms. Exposure to aerosol from marijuana with and without cannabinoids impaired FMD by ~50%. FMD was similarly impaired by aerosols from Yocan, and from Volcano at both its standard temperature (185°C) and the minimum sublimation temperature of THC (157°C), even though the low-temperature aerosol condition did not effectively deliver THC to the circulation. Modeled secondhand exposure based on diluted Volcano aerosol also impaired FMD. FMD was not affected in rats exposed to clean air or water vapor passed through the Volcano system.
Conclusions Acute direct exposure and modeled secondhand exposure to marijuana leaf vaporizer aerosol, regardless of cannabinoid concentration or aerosol generation temperature, impair endothelial function in rats comparably to marijuana smoke. Our findings indicate that use of leaf vaporizers is unlikely to reduce vascular risk burden of smoking marijuana.
README
Impairment of Endothelial Function by Aerosol from Marijuana Leaf Vaporizers
https://doi.org/10.5061/dryad.4xgxd25gv
Description of methods used for collection/generation of data: We obtained raw ultrasound data for femoral artery images. We have included the images in primary zip folders. Each primary zip folder includes many subfolders. Each subfolder has 20 records over time, which is corresponding to each animal at each time point and smoke conditions. Time points include pre- and post-smoke exposure. Each time point contains 10 records which include baseline and post-transient ischemia (from 0 min to 5 min).
Methods for processing the data: For femoral artery diameter measurements, we used an automated system (Brachial Analyzer; Medical Imaging Applications, Coralville, IA) to measure diameters over time.
Some of the raw data on which Figure 1 is based has been lost and is not recoverable. This is regrettable but note that the interpretations and conclusions from this experiment are nonetheless validated by the subsequent experiments, for which all of the raw data are provided.
Methods
For FMD data, raw ultrasound data were collected by an ultrasound transducer (Vevo 660 or Vevo 3100 LT; VisualSonics, Totonto, Canada). Ultrasound images were processed using an automated system (Brachial Analyzer; Medical Imaging Applications, Coralville, IA).