Data from: Morphological and molecular characterization within 26 strains of the genus Cylindrospermum (Nostocaceae, Cyanobacteria), with descriptions of three new species
Johansen, Jeffrey R., University of South Bohemia in České Budějovice, John Carroll University
Bohunicka, Marketa, University of South Bohemia in České Budějovice, Academy of Sciences of the Czech Republic
Lukesova, Alena, Academy of Sciences of the Czech Republic
Hreckova, Kristyna, University of South Bohemia in České Budějovice, Academy of Sciences of the Czech Republic
Vaccarino, Melissa A., John Carroll University
Chesarino, Nicholas M., John Carroll University
Published Oct 16, 2017 on Dryad.
Cite this dataset
Johansen, Jeffrey R. et al. (2017). Data from: Morphological and molecular characterization within 26 strains of the genus Cylindrospermum (Nostocaceae, Cyanobacteria), with descriptions of three new species [Dataset]. Dryad. https://doi.org/10.5061/dryad.50pg2
Twenty-six strains morphologically identified as Cylindrospermum as well as the closely related taxon Cronbergia siamensis were examined microscopically as well as phylogenetically using sequence data for the 16S rRNA gene and the 16S-23S ITS region. Phylogenetic analysis of the 16S rRNA revealed three distinct clades. The clade we designate as Cylindrospermum sensu stricto had all five of the foundational species, C. maius, C. stagnale, C. licheniforme, C. muscicola, and C. catenatum. In addition to these taxa, three species new to science in this clade were described: C. badium, C. moravicum, and C. pellucidum. Our evidence indicates that Cronbergia is a later synonym of Cylindrospermum. The phylogenetic position of Cylindrospermum within the Nostocaceae was not clearly resolved in our analyses. Cylindrospermum is unusual among cyanobacterial genera in that the morphological diversity appears to be more evident than sequence divergence. Taxa were clearly separable using morphology, but had very high percent similarity among ribosomal sequences. Given the high diversity we noted in this study, we conclude that there is likely much more diversity remaining to be described in this genus.
Alignment for 16S-23S ITS regions containing both tRNA genes for Cylindrospermum
This is a nexus alignment for 8 species of Cylindrospermum plus out group Aulosira bohemensis (13 OTUs in all) for the 16S-23S ITS region in the operons containing both tRNA genes in the ITS. Alignment was performed manually using secondary structure of conserved domains for the alignment. Sequences between conserved domains were aligned with ClustalW.
Johansen et al 2013_ITS_both_tRNA.nex
Alignment of 16S-23S ITS region for operons containing no tRNA genes
Alignment for 10 species (14 OTUs) of the sequence for the 16S-23S ITS region for operons lacking tRNA genes. Alignment was performed manually using secondary structure of the conserved domains as a guide. Spaces between conserved domains were aligned using ClustalW.