Data for: Low protease activity in B cell follicles promotes retention of intact antigens after immunization
Data files
Mar 15, 2023 version files 1.70 GB
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Fig1E_Outside_Follicles_6hrs_Antigen_Signal.tif
3.15 MB
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Fig1E_Outside_Follicles_6hrs_FRET_Signal.tif
610.34 KB
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Fig1E_Outside_Follicles_Day2_Antigen_Signal.tif
3.15 MB
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Fig1E_Outside_Follicles_Day2_FRET_Signal.tif
339.44 KB
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Fig1F_Inside_Follicles_Day2_Antigen_Signal.tif
545.24 KB
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Fig1F_Inside_Follicles_Day2_FRET_Signal.jpg
70.78 KB
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Fig1F_Inside_Follicles_Day3_Antigen_Signal.tif
3.15 MB
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Fig1F_Inside_Follicles_Day3_FRET_Signal.tif
250.86 KB
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Fig2A_Site1_eOD_CellMembrane.tif
413.58 KB
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Fig2A_Site1_FRET_Overlay.jpg
142.73 KB
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Fig2A_Site2_eOD_CellMembrane.tif
413.58 KB
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Fig2A_Site2_FRET_Overlay.jpg.jpg
87.84 KB
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Fig3A_ADAM10_HighMag_Protease_FDC.tif
3.89 MB
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Fig3A_ADAM10_LowMag_Protease_FDC.tif
71.74 MB
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Fig3A_ADAM17_HighMag_Protease_FDC.tif
3.10 MB
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Fig3A_ADAM17_LowMag_Protease_FDC.tif.tif
63.25 MB
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Fig3A_Isotype_HighMag_Isotype_FDC.tif
691.42 KB
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Fig3A_Isotype_LowMag_Isotype_FDC.tif
25.77 MB
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Fig3A_MMP14_HighMag_Protease_FDC.tif
7.93 MB
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Fig3A_MMP14_LowMag_Protease_FDC.tif
101.58 MB
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Fig3A_MMP9_HighMag_Protease_FDC.tif
470.67 KB
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Fig3A_MMP9_LowMag_Protease_FDC.tif
19.25 MB
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Fig3B_ADAM10.tif
325.41 KB
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Fig3B_ADAM17.tif
325.41 KB
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Fig3B_CD169.tif
325.41 KB
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Fig3B_LYVE1.tif
325.41 KB
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Fig3B_MMP14.tif
325.41 KB
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Fig3C_Site1_eOD_60mer.tif
62.41 KB
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Fig3C_Site1_Proease.tif
60.87 KB
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Fig3C_Site1_Stability.tif
196.91 KB
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Fig3C_Site2_eOD_60mer.tif
151.56 KB
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Fig3C_Site2_Protease.tif
50.68 KB
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Fig3C_Site2_Stability.jpg
615.11 KB
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Fig4B_HighMag_Inside_Follicles_Site1_CD35_AZP.tif
454.75 KB
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Fig4B_HighMag_Inside_Follicles_Site1_PolyR.tif
454.74 KB
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Fig4B_HighMag_Inside_Follicles_Site2_CD35_AZP.tif
473.08 KB
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Fig4B_HighMag_Inside_Follicles_Site2_PolyR.tif
473.08 KB
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Fig4B_HighMag_Outside_Follicles_Site1_CD35_AZP.tif
432.84 KB
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Fig4B_HighMag_Outside_Follicles_Site1_PolyR.tif
432.84 KB
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Fig4B_HighMag_Outside_Follicles_Site2_CD35_AZP.tif
447.44 KB
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Fig4B_HighMag_Outside_Follicles_Site2_PolyR.tif
447.44 KB
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Fig4B_LowMag_Immunized_AZP.tif
10.21 MB
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Fig4B_LowMag_Immunized_CD35.tif
10.21 MB
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Fig4F_MMP9.tif
293.50 KB
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Fig4F_Trypsin.tif
264.93 KB
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Fig4F_Untreated.tif
1.05 MB
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Fig4I_mCherry_ATBcells_60mer.tif
3.90 MB
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Fig4I_mCherry_FDC_60mer.tif
3.90 MB
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Fig4I_MMP9_ATBcells_60mer.tif
4.29 MB
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Fig4I_MMP9_FDC_60mer.tif
3.88 MB
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Fig5B_Bolus-Trimer_Day1_Site1.tif
621.29 KB
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Fig5B_Bolus-Trimer_Day1_Site2.tif
795.79 KB
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Fig5B_Bolus-Trimer_Day2_Site1.tif
798.87 KB
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Fig5B_Bolus-Trimer_Day2_Site2.tif
654.54 KB
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Fig5B_Bolus-TrimerNP_Day1_Site1.tif
868.55 KB
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Fig5B_Bolus-TrimerNP_Day1_Site2.tif
741.88 KB
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Fig5B_Bolus-TrimerNP_Day2_Site1.tif
688.19 KB
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Fig5B_Bolus-TrimerNP_Day2_Site2.tif
559.74 KB
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Fig5B_ED-TrimerNP_Day14_Site1.tif
3.15 MB
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Fig5B_ED-TrimerNP_Day14_Site2.tif
336.89 KB
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Fig6J_aCD35eOD_eOD.tif
1.24 MB
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Fig6J_aCD35eOD_FDC.tif
1.24 MB
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Fig6J_IsoeOD_eOD.tif
1.24 MB
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Fig6J_IsoeOD_FDC.tif
1.24 MB
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FigS1D_PostBleach_Acceptor.tif
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FigS1D_PostBleach_Donor.tif
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FigS1D_PreBleach_Acceptor.tif
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FigS1D_PreBleach_Donor.tif
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FigS1E_PostBleach_Acceptor.tif
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FigS1E_PostBleach_Donor.tif
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FigS1E_PreBleach_Acceptor.tif
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FigS1E_PreBleach_Donor.tif
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FigS1F_PostBleach_Acceptor.tif
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FigS1F_PostBleach_Donor.tif
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FigS1F_PreBleach_Acceptor.tif
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FigS1F_PreBleach_Donor.tif
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FigS1G_PostBleach_Acceptor.tif
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FigS1G_PostBleach_Donor.tif
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FigS1G_PreBleach_Acceptor.tif
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FigS1G_PreBleach_Donor.tif
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FigS2B_Inside_follicle_eOD-60mer.tif
1.08 MB
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FigS2B_Inside_follicle_IgD_eOD-60mer.tif
1.08 MB
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FigS2B_Interfollicle_eOD-60mer.tif
506.98 KB
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FigS2B_Interfollicle_IgD_eOD-60mer.tif
506.98 KB
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FigS2D_Day1_FDC_eOD60mer.tif
1.32 MB
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FigS2D_Day2_FDC_eOD60mer.tif.tif
846.10 KB
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FigS2D_Day3_FDC_eOD60mer.tif
865.33 KB
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FigS2F_6hrs_FDC.tif
635.02 KB
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FigS2F_6hrs_HA-Fe.tif
635.02 KB
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FigS2F_Day_2_FDC.tif
475.43 KB
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FigS2F_Day_2_HA-Fe.tif
475.43 KB
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FigS3A_6_hr_Outside_Follicle_eOD-60mer.tif
236.02 KB
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FigS3A_6_hr_Outside_Follicle_VRC01_Ab.tif
702.99 KB
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FigS3A_Day_1_Outside_Follicle_eOD-60mer.tif
3.15 MB
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FigS3A_Day_1_Outside_Follicle_VRC01_Ab.tif
3.15 MB
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FigS3A_Day_2_Outside_Follicle_eOD-60mer.tif
3.15 MB
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FigS3A_Day_2_Outside_Follicle_VRC01_Ab.tif
3.15 MB
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FigS3A_Day_3_Inside_Follicle_eOD-60mer.tif
3.15 MB
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FigS3A_Day_3_Inside_Follicle_FDC.tif
3.15 MB
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FigS3A_Day_3_Inside_Follicle_VRC01_Ab.tif
3.15 MB
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FigS3B_eOD-60mer.tif
2.91 MB
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FigS3B_Isotype_Control.tif
2.91 MB
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FigS3C_eOD-60mer.tif
714.65 KB
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FigS3C_VRC01_Ab.tif
714.65 KB
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FigS6A_Immunized_ADAM17_FDC.tif
89.67 MB
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FigS6A_Immunized_ADAM17_Protease.tif
89.67 MB
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FigS6B_Immunized_ADAM10_FDC.tif
2.95 MB
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FigS6B_Immunized_ADAM10_Protease.tif
2.95 MB
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FigS6B_Immunized_MMP14_FDC.tif
1.01 MB
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FigS6B_Immunized_MMP14_Protease.tif
1.01 MB
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FigS6B_Immunized_MMP9_FDC.tif
1.14 MB
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FigS6B_Immunized_MMP9_Protease.tif
1.14 MB
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FigS6C_ADAM10.tif
585.34 KB
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FigS6C_ADAM17.tif
585.34 KB
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FigS6C_CD169.tif
585.34 KB
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FigS6C_LYVE1.tif
585.34 KB
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FigS6C_MMP14.tif
585.34 KB
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FigS6E_humanTonsil_ADAM10_FDC.tif
37.48 MB
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FigS6E_humanTonsil_ADAM10_Protease.tif
37.48 MB
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FigS6E_humanTonsil_ADAM17_FDC.tif
165.33 MB
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FigS6E_humanTonsil_ADAM17_Protease.tif
165.33 MB
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FigS6E_humanTonsil_Isotype_FDC.tif
122.50 MB
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FigS6E_humanTonsil_Isotype_Isotype_Control.tif
122.50 MB
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FigS6E_humanTonsil_MMP14_FDC.tif
42.84 MB
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FigS6E_humanTonsil_MMP14_Protease.tif
42.84 MB
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FigS6E_humanTonsil_MMP9_FDC.tif
126.83 MB
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FigS6E_humanTonsil_MMP9_Protease.tif
126.83 MB
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FigS7B_CD169_eOD60mer_Colocalization.tif
1.19 MB
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FigS7B_CD169_eOD60mer.tif
1.15 MB
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FigS7B_LYVE1_eOD60mer_Colocalization.tif
2.24 MB
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FigS7B_LYVE1_eOD60mer.tif
1.22 MB
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FigS7D_Ag_Adj_ADAM10_eOD-60mer.tif
1.61 MB
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FigS7D_Ag_Adj_ADAM10_Protease.tif
1.61 MB
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FigS7D_Ag_Adj_ADAM17_eOD-60mer.tif
4.20 MB
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FigS7D_Ag_Adj_ADAM17_protease.tif
4.20 MB
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FigS7D_Ag_Adj_MMP14_eOD-60mer.tif
2.54 MB
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FigS7D_Ag_Adj_MMP14_Protease.tif
2.54 MB
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FigS7D_Ag_only_ADAM10_eOD-60mer.tif
3.15 MB
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FigS7D_Ag_only_ADAM10_Protease.tif
3.15 MB
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FigS7D_Ag_only_ADAM17_eOD-60mer.tif
3.15 MB
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FigS7D_Ag_only_ADAM17_Protease.tif
3.15 MB
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FigS7D_Ag_only_MMP14_eOD-60mer.tif
4.41 MB
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FigS7D_Ag_only_MMP14_Protease.tif
4.41 MB
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FigS7F_Naive_AZP.tif
11.84 MB
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FigS7F_Naive_FDC.tif
11.84 MB
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FigS7F_NaiveLN_Inside_FDC_Site1_AZP_FDC.tif
597.09 KB
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FigS7F_NaiveLN_Inside_FDC_Site1_PolyR_AZP.tif
596.97 KB
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FigS7F_NaiveLN_Inside_FDC_Site2_FDC_AZP.tif
599.77 KB
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FigS7F_NaiveLN_Inside_FDC_Site2_PolyR_AZP.tif
599.65 KB
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FigS7F_NaiveLN_OutsideFDC_Site1_AZP.tif
597.09 KB
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FigS7F_NaiveLN_OutsideFDC_Site1_PolyR_AZP.tif
596.97 KB
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FigS7F_NaiveLN_OutsideFDC_Site2_AZP.tif
597.09 KB
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FigS7F_NaiveLN_OutsideFDC_Site2_PolyR_AZP.tif
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FigS7G_D-Isomer_CD35.tif
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README.md
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Abstract
The structural integrity of vaccine antigens is critical to the generation of protective antibody responses, but the impact of protease activity on vaccination in vivo is poorly understood. We characterized protease activity in lymph nodes and found that antigens were rapidly degraded in the subcapsular sinus, paracortex, and interfollicular regions, whereas low protease activity and antigen degradation rates were detected in the vicinity of follicular dendritic cells (FDCs). Correlated with these findings, immunization regimens designed to target antigen to FDCs led to germinal centers dominantly targeting intact antigen, whereas traditional immunizations led to much weaker responses that equally targeted the intact immunogen and antigen breakdown products. Thus, spatially compartmentalized antigen proteolysis affects humoral immunity and can be exploited.
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