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Pollen color morphs take different paths to fitness

Citation

Koski, Matthew; Berardi, Andrea; Galloway, Laura (2020), Pollen color morphs take different paths to fitness, Dryad, Dataset, https://doi.org/10.5061/dryad.547d7wm4m

Abstract

Color phenotypes are often involved in communication and are thus under selection by species interactions. However, selection may also act on color through correlated traits or alternative functions of biochemical pigments. Such forms of selection are instrumental in maintaining petal color diversity in plants. Pollen color also varies markedly, but the maintenance of this variation is little understood. In Campanula americana, pollen ranges from white to dark purple, with darker morphs garnering more pollinator visits and exhibiting elevated pollen performance under heat stress. Here, we generate an F2 population segregating for pollen color and measure correlations with floral traits, pollen attributes, and plant-level traits related to fitness. We determine the pigment biochemistry of color variants, and evaluate maternal and paternal fitness of light and dark morphs by crossing within and between morphs. Pollen color was largely uncorrelated with floral traits (petal color, size, nectar traits) suggesting it can evolve independently. Darker pollen grains were larger and had higher anthocyanin content (cyanidin and peonidin) which may explain why they outperform light pollen under heat stress. Overall, pollen-related fitness metrics were greater for dark pollen, and dark pollen sires generated seeds with higher germination potential. Conversely, light pollen plants produce 61% more flowers than dark, and 18% more seeds per fruit, suggesting a seed production advantage. Results indicate that light and dark morphs may achieve fitness through different means—dark morphs appear to have a pollen advantage while light morphs have an ovule advantage—helping to explain the maintenance of pollen color variation.

Usage Notes

Datasets are uploaded in a single excel file with unique tabs for each dataset. Each dataset is associated with a 'header' tab to describe the column headers. Missing data are denoted by 'NA' or '.'. 

F2Phenotypes: Data used to generated phenotypic correlations between pollen color and various plant attributes. 

PollenChem: HPLC results of flavones, flavonols and anthocyanins in the 5 different pollen color classes. 

SeedSetData: Seed production of light-pollen and dark-pollen plants when crossed by either light or dark pollen. 

F3Fitness: Seed germination, 

The 'PollenChem' Tab does not have an associated tab to describe column headers. These are HPLC results for each of 5 pollen color classes, with each header displaying the compound and values in ug/mL