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Data from quantitative real-time PCR of corticotropin-releasing hormone and glucocorticoid receptor in the hippocampus and hypothalamus of rats subjected to midline fluid percussion injury or control sham surgery.

Citation

Rowe, Rachel et al. (2020), Data from quantitative real-time PCR of corticotropin-releasing hormone and glucocorticoid receptor in the hippocampus and hypothalamus of rats subjected to midline fluid percussion injury or control sham surgery., Dryad, Dataset, https://doi.org/10.5061/dryad.547d7wm63

Abstract

These files contain all data from quantitative real-time PCR of corticotropin-releasing hormone (CRH) and glucocorticoid receptor (GR) measured in the hippocampus and hypothalamus of male and female rats. Rats were randomly assigned to a treatment group before the initiation of the study. Rats received a control sham surgery or were subjected to midline fluid percussion injury to induce a diffuse traumatic brain injury. Tissue biopsies were collected at 7 days post-injury and analyzed via quantitative real-time PCR. 

 

Methods

See manuscript for full description of methods.

Usage Notes

Correspondence:

Theresa Currier Thomas

theresathomas@email.arizona.edu

Associate Professor

Translational Neurotrauma Research Program

Barrow Neurological Institute at Phoenix Children's Hospital

Department of Child Health, University of Arizona College of Medicine-Phoenix

425 N. 5th St. | 320 ABC-1 Building

Phoenix, Arizona 85004-2127

 

File List

CRH_Hippocampus.csv

CRH_Hypothalamus.csv

GR_Hippocampus.csv

GR_Hypothalamus.csv

 

Description

These files contain all data from quantitative real-time PCR of corticotropin-releasing hormone (CRH) and glucocorticoid receptor (GR) measured in the hippocampus and hypothalamus of male and female rats. Rats were randomly assigned to a treatment group before the initiation of the study. Rats received a control sham surgery or were subjected to midline fluid percussion injury to induce a diffuse traumatic brain injury. Tissue biopsies were collected at 7 days post-injury and analyzed via quantitative real-time PCR. 

File Descriptions

CRH_Hippocampus.csv:  File containing all data from quantitative real-time PCR of corticotropin-releasing hormone (CRH) measured in the hippocampus of male and female rats at 7 days after traumatic brain injury via midline fluid percussion injury or a control sham surgery.

  1. ID – Unique identifier for an individual rat that reflects the study number of the rat, the sex of the rat (665=male, 666=female), and indicates cage mates using a letter (A or B); for example, rat 665993A and 665993B were study number 993, male rats that were housed in the same cage.
  2. Treatment – Categorical variable denoting whether each rat received a traumatic brain injury (Injury) via midline fluid percussion or a control sham surgery (Sham).
  3. Sex – Categorical variable denoting the sex of each rat; male (M) or female (F).
  4. Raw_CT1_CRH – Raw value of the cycle threshold of CRH for sample 1 of the rat.
  5. Raw_CT2_CRH – Raw value of the cycle threshold of CRH for sample 2 of the rat.
  6. Raw_CT3_CRH – Raw value of the cycle threshold of CRH for sample 3 of the rat.
  7. Raw_CT1_18s_rRNA – Raw value of the cycle threshold of the biological control of Eukaryotic 18S ribosomal RNA from sample 1.
  8. Raw_CT2_18s_rRNA – Raw value of the cycle threshold of the biological control of Eukaryotic 18S ribosomal RNA from sample 2.
  9. Raw_CT3_18s_rRNA Raw value of the cycle threshold of the biological control of Eukaryotic 18S ribosomal RNA from sample 3.
  10. dCT1– The difference between the cycle threshold value for CRH and the cycle threshold value for the 18s rRNA for sample 1.
  11. dCT2– The difference between the cycle threshold value for CRH and the cycle threshold value for the 18s rRNA for sample 2.
  12. dCT3– The difference between the cycle threshold value for CRH and the cycle threshold value for the 18s rRNA for sample 3.
  13. CT_Mean_CRH – Mean of the triplicate cycle threshold values of CRH.
  14. CT_Mean_18s_rRNA – Mean of the triplicate cycle threshold values of the biological control of Eukaryotic 18S ribosomal RNA.
  15. dCT_Mean – The difference between the mean cycle threshold value for CRH and the mean cycle threshold value for the 18s rRNA.
  16. ddCT – dCT_Mean normalized to the average of the same sex sham.
  17. Fold_Change – The relative gene expression of CRH calculated using the comparative CT method where the fold change of CRH is calculated using the equation: 2^(-ddCT).

CRH_Hypothalamus.csv:  File containing all data from quantitative real-time PCR of corticotropin-releasing hormone (CRH) measured in the hypothalamus of male and female rats at 7 days after traumatic brain injury via midline fluid percussion injury or a control sham surgery.

  1. ID – Unique identifier for an individual rat that reflects the study number of the rat, the sex of the rat (665=male, 666=female), and indicates cage mates using a letter (A or B); for example, rat 665993A and 665993B were study number 993, male rats that were housed in the same cage.
  2. Treatment – Categorical variable denoting whether each rat received a traumatic brain injury (Injury) via midline fluid percussion or a control sham surgery (Sham).
  3. Sex – Categorical variable denoting the sex of each rat; male (M) or female (F).
  4. Raw_CT1_CRH – Raw value of the cycle threshold of CRH for sample 1 of the rat.
  5. Raw_CT2_CRH – Raw value of the cycle threshold of CRH for sample 2 of the rat.
  6. Raw_CT3_CRH – Raw value of the cycle threshold of CRH for sample 3 of the rat.
  7. Raw_CT1_18s_rRNA – Raw value of the cycle threshold of the biological control of Eukaryotic 18S ribosomal RNA from sample 1.
  8. Raw_CT2_18s_rRNA – Raw value of the cycle threshold of the biological control of Eukaryotic 18S ribosomal RNA from sample 2.
  9. Raw_CT3_18s_rRNA – Raw value of the cycle threshold of the biological control of Eukaryotic 18S ribosomal RNA from sample 3.
  10. dCT1– The difference between the cycle threshold value for CRH and the cycle threshold value for the 18s rRNA for sample 1.
  11. dCT2– The difference between the cycle threshold value for CRH and the cycle threshold value for the 18s rRNA for sample 2.
  12. dCT3–The difference between the cycle threshold value for CRH and the cycle threshold value for the 18s rRNA for sample 3.
  13.  CT_Mean_CRH – Mean of the triplicate cycle threshold values of CRH.
  14. CT_Mean_18s_rRNA – Mean of the triplicate cycle threshold values of the biological control of Eukaryotic 18S ribosomal RNA.
  15. dCT_Mean – The difference between the mean cycle threshold value for CRH and the mean cycle threshold value for the 18s rRNA.
  16. ddCT – dCT_Mean normalized to the average of the same sex sham.
  17. Fold_Change – The relative gene expression of CRH calculated using the comparative CT method where the fold change of CRH is calculated using the equation: 2^(-ddCT).

 

 

 

 

GR_Hippocampus.csv:  File containing all data from quantitative real-time PCR of glucocorticoid receptor (GR) measured in the hippocampus of male and female rats at 7 days after traumatic brain injury via midline fluid percussion injury or a control sham surgery.

  1. ID – Unique identifier for an individual rat that reflects the study number of the rat, the sex of the rat (665=male, 666=female), and indicates cage mates using a letter (A or B); for example, rat 665993A and 665993B were study number 993, male rats that were housed in the same cage.
  2. Treatment – Categorical variable denoting whether each rat received a traumatic brain injury (Injury) via midline fluid percussion or a control sham surgery (Sham).
  3. Sex – Categorical variable denoting the sex of each rat; male (M) or female (F).
  4. Raw_CT1_GR – Raw value of the cycle threshold of GR for sample 1 of the rat.
  5. Raw_CT2_GR – Raw value of the cycle threshold of GR for sample 2 of the rat.
  6. Raw_CT3_GR – Raw value of the cycle threshold of GR for sample 3 of the rat.
  7. Raw_CT1_18s_rRNA – Raw value of the cycle threshold of the biological control of Eukaryotic 18S ribosomal RNA from sample 1.
  8. Raw_CT2_18s_rRNA – Raw value of the cycle threshold of the biological control of Eukaryotic 18S ribosomal RNA from sample 2.
  9. Raw_CT3_18s_rRNA – Raw value of the cycle threshold of the biological control of Eukaryotic 18S ribosomal RNA from sample 3.
  10. dCT1– The difference between the cycle threshold value for GR and the cycle threshold value for the 18s rRNA for sample 1.
  11. dCT2– The difference between the cycle threshold value for GR and the cycle threshold value for the 18s rRNA for sample 2.
  12. dCT3–The difference between the cycle threshold value for GR and the cycle threshold value for the 18s rRNA for sample 3.
  13. CT_Mean_GR – Mean of the triplicate cycle threshold values of GR.
  14. CT_Mean_18s_rRNA – Mean of the triplicate cycle threshold values of the biological control of Eukaryotic 18S ribosomal RNA.
  15. dCT_Mean – The difference between the mean cycle threshold value for GR and the mean cycle threshold value for the 18s rRNA.
  16. ddCT – dCT_Mean normalized to the average of the same sex sham.
  17. Fold_Change – The relative gene expression of GR calculated using the comparative CT method where the fold change of GR calculated using the equation: 2^(-ddCT).

 

GR_Hypothalamus.csv:  File containing all data from quantitative real-time PCR of glucocorticoid receptor (GR) measured in the hypothalamus of male and female rats at 7 days after traumatic brain injury via midline fluid percussion injury or a control sham surgery.

  1. ID – Unique identifier for an individual rat that reflects the study number of the rat, the sex of the rat (665=male, 666=female), and indicates cage mates using a letter (A or B); for example, rat 665993A and 665993B were study number 993, male rats that were housed in the same cage.
  2. Treatment – Categorical variable denoting whether each rat received a traumatic brain injury (Injury) via midline fluid percussion or a control sham surgery (Sham).
  3. Sex – Categorical variable denoting the sex of each rat; male (M) or female (F).
  4. Raw_CT1_GR – Raw value of the cycle threshold of GR for sample 1 of the rat.
  5. Raw_CT2_GR – Raw value of the cycle threshold of GR for sample 2 of the rat.
  6. Raw_CT3_GR – Raw value of the cycle threshold of GR for sample 3 of the rat.
  7. Raw_CT1_18s_rRNA – Raw value of the cycle threshold of the biological control of Eukaryotic 18S ribosomal RNA from sample 1.
  8. Raw_CT2_18s_rRNA – Raw value of the cycle threshold of the biological control of Eukaryotic 18S ribosomal RNA from sample 2.
  9. Raw_CT3_18s_rRNA – Raw value of the cycle threshold of the biological control of Eukaryotic 18S ribosomal RNA from sample 3.
  10. dCT1– The difference between the cycle threshold value for GR and the cycle threshold value for the 18s rRNA for sample 1.
  11. dCT2– The difference between the cycle threshold value for GR and the cycle threshold value for the 18s rRNA for sample 2. 
  12. dCT3–The difference between the cycle threshold value for GR and the cycle threshold value for the 18s rRNA for sample 3.
  13. CT_Mean_GR – Mean of the triplicate cycle threshold values of GR.
  14. CT_Mean_18s_rRNA – Mean of the triplicate cycle threshold values of the biological control of Eukaryotic 18S ribosomal RNA.
  15. dCT_Mean – The difference between the mean cycle threshold value for GR and the mean cycle threshold value for the 18s rRNA.
  16. ddCT – dCT_Mean normalized to the average of the same sex sham.
  17. Fold_Change – The relative gene expression of CRH calculated using the comparative CT method where the fold change of GR is calculated using the equation: 2^(-ddCT).

 

Funding

Valley Research Partnership, Award: VRP 2256030

Arizona Biomedical Research Commission, Award: ADHS14- 00003606

National Institutes of Health, Award: R01NS100793

Valley Research Partnership, Award: VRP 2256030