Data from: A technical note on variable inter-frame interval as a cause of non-physiological experimental artefacts in ultrasound
Miguez, Diego, Manchester Metropolitan University
Hodson-Tole, Emma F., Manchester Metropolitan University
Loram, Ian D.
Harding, Peter J., Manchester Metropolitan University
Published Apr 28, 2017 on Dryad.
Cite this dataset
Miguez, Diego; Hodson-Tole, Emma F.; Loram, Ian D.; Harding, Peter J. (2017). Data from: A technical note on variable inter-frame interval as a cause of non-physiological experimental artefacts in ultrasound [Dataset]. Dryad. https://doi.org/10.5061/dryad.550sg
Ultrasound imaging is a well-recognised technique for the study of static tissues but its suitability for studying tissue dynamics depends upon accurate frame time information, which may not always be available to users. Here we present methods to quantify the inter-frame interval (IFI) variability, and evaluate different procedures for collecting temporal information from two ultrasound-imaging devices. The devices tested exhibited variable IFIs that could only be confirmed by direct measures of timing signals, available by means of electrical signals (triggers) and/or temporal information contained in the software used for the ultrasound data collection. Interpolating frame-by-frame measures of dynamic changes within image sequences using individual IFI values provided improved synchronisation between measures of skeletal muscle movement and activation; validating ultrasound as a valuable technique for the study of musculoskeletal tissue dynamics, when correctly implemented.
Full description of the uploaded data files
Effects of variable interframe interval on data synchronisation
Results of analysis of ultrasound image sequence, recorded during low level electrical stimulation of motor nerve. Including associated frame time and electromyographic data. Full details available in the ReadMe file.
Frame times from two timestamping approaches
Frame times recorded from ultrasound imaging device using hardware and software timestamping techniques. Full details available in the ReadMe file.
Software recorded interframe intervals
Inter-frame intervals from ultrasound imaging device, recorded using software timestamping approach described in the manuscript. Full details of file contents are described in the ReadMe file.
Ultrasound video skeletal muscle movements
B-mode ultrasound video of lower leg muscles of one participant, recorded (approx. 80 fps) using a LS128 (Telemed, Ultrasound Medical Systems, Italy) ultrasound imaging device. The video was recorded whil low amplitude (12 mA) electrical stimulation was delivered (1 Hz) to the motor nerve (Tibial nerve).