Data from: Environmentally relevant concentrations of bifenthrin affect the expression of estrogen and glucocorticoid receptors in brains of female western mosquitofish
Ligocki, Isaac Y.
Connon, Richard E.
Calisi, Rebecca M.
Published Aug 26, 2019 on Dryad.
Cite this dataset
Ligocki, Isaac Y. et al. (2019). Data from: Environmentally relevant concentrations of bifenthrin affect the expression of estrogen and glucocorticoid receptors in brains of female western mosquitofish [Dataset]. Dryad. https://doi.org/10.5061/dryad.5888cm4
In recent decades, pyrethroid pesticides have been deemed a safer alternative to previously used pesticides. While some evidence supports this assumption in mammals and birds, exposure to certain pyrethroids can affect concentrations of hormones vital to reproduction in fish. Thus, we hypothesized that pyrethroid exposure impacts fish reproductive behavior and the expression of genes associated with reproduction. We tested our hypothesis by examining effects of the widely used pyrethroid pesticide, bifenthrin, on the reproductive behaviors of the broadly distributed livebearing western mosquitofish, Gambusia affinis. We exposed sexually mature female fish to one of five environmentally relevant concentrations of bifenthrin and conducted behavioral assays to assess reproductive, social, and space use behaviors before and after exposure. We did not detect changes in behaviors measured in response to bifenthrin. However, exposure was associated with increased expression of an estrogen receptor gene (ER-α) and glucocorticoid receptor (GR) in brain tissue at bifenthrin concentrations at concentrations of 5.90 and 24.82 ng/L, and 5.90 and 12.21 ng/L, respectively. Our study supports the perspective that the use of multiple endpoints through integrative approaches is essential for understanding the cumulative impact of pollutants. Integrating physiological, morphological, and behavioral investigations of nonlethal concentrations of pollutants like bifenthrin may heighten our potential to predict their impact on individuals, populations, and communities.
delta Ct values for focal genes
This file contains Delta Ct values for gene expression used in subsequent analysis. Each row represents one individual fish, "Treatment" indicates the measured concentration of bifenthrin in each treatment group. Gcliv - indicates glucocorticoid receptor (liver), ChorLliv - indicates Choriogenin L receptor (liver), VtgCliv - indicates Vitellogenin C (liver), Erabrn - indicates Estrogen receptor alpha (brain), Arabrn - indicates Androgen receptor (brain), Cyp19brn indicates Cyp19 receptor (brain), GCbrn - indicates Glucocorticoid receptor (brain), NS1brn - indicates neuroserpin 1 receptor (brain).
Mate Choice Data
Time spent with male Gambusia affinis in mate choice test. All times are in seconds.