1. Seasonal changes in the environment, such as varying temperature, have the potential to change the functional relationship between ectothermic animals, such as insects, and their microbiomes. Our objectives were to determine: a) whether seasonal changes in temperature shift the composition of the insect gut microbiome, and b) if changes in the microbiome are concomitant with changes in the physiology of the host, including the immune system and response to cold. 2. We exposed laboratory populations of the spring field cricket, Gryllus veletis (Orthoptera: Gryllidae), to simulated overwintering winter and spring, we extracted and sequenced 16S bacterial genomic DNA from cricket guts, to capture seasonal variation in the composition of the microbiome. 3. The composition of the gut microbiome was similar between microcosms, and overall me, where overwintering resulted in permanent changes to these microbial communities. In particular, the abundance of Pseudomonas spp. decreased, and that of Wolbachia spp. increased, during overwintering. 4. Concurrent with overwintering changes in the gut microbiome, G. veletis acquire freeze-tolerance and immune function shifts temporarily, returning to summer levels of activity in the spring. Specifically, haemocyte concentrations increase but survival of fungal infection decreases in the winter, whereas the ability to clear bacteria from the haemolymph remains unchanged. 5. Overall, we demonstrate that the gut microbiome does shift seasonally, and in concert with other physiological changes. We hypothesize that these changes may be linked, and suggest that it will next be important to determine if these changes in the microbiome contribute to host overwintering success.