This README file was generated on December 21, 2023 by Ryley Yost.

GENERAL INFORMATION

1. Title of Dataset: Recovery from social isolation requires dopamine in males, but not the autism-related gene nlg3 in either sex.

2. Author Information
   A. Principal Investigator Contact Information (Corresponding Author)
   Name: Anne Simon
   Institution: Western University
   Address: London, ON, Canada
   Email: asimon28@uwo.ca

   B. Associate or Co-investigator Contact Information
   Name: Ryley Yost
   Institution: Western University
   Address: London, ON, Canada
   Email: ryost@uwo.ca

3. Date of data collection (single date, range, approximate date): 2017-2023

4. Geographic location of data collection: London, ON, Canada & Hamilton, ON, Canada

5. Information about funding sources that supported the collection of the data: NSERC DG 05054-2022, NSERC DG 05420-2019.

SHARING/ACCESS INFORMATION

1. Licenses/restrictions placed on the data: None

2. Links to publications that cite or use the data:

Yost, Ryley T, Scott, Andrew M, Kurbaj, Judy M, Walshe-Roussel, Brendan, Dukas, Reuven, Simon, Anne F. (Forthcoming 2023-2024). Recovery from social isolation requires dopamine in males, but not the autism-related gene nlg3 in either sex.

1. Links to other publicly accessible locations of the data: None

2. Links/relationships to ancillary data sets: None

3. Was data derived from another source? No
   A. If yes, list source(s): NA

4. Recommended citation for this dataset:

Yost, Ryley T et al. (Forthcoming 2023-2024). Data from: Recovery from social isolation requires dopamine in males, but not the autism-related gene nlg3 in either sex [Dataset]. Dryad. https://doi.org/10.5061/dryad.59zw3r2fs.

DATA & FILE OVERVIEW

1. File List:

A) Figure_1.xls
B) Figure_2.xls
C) Figure_3.xls
D) Figure_4.xls
E) Figure_5.xls
F) Supplemental_Figure_3.xls

1. Relationship between files, if important: None

2. Additional related data collected that was not included in the current data package: None

3. Are there multiple versions of the dataset? No
   A. If yes, name of file(s) that was updated: NA
   i. Why was the file updated? NA
   ii. When was the file updated? NA

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DATA-SPECIFIC INFORMATION FOR: Figure_1.xls

Specific graphs (Figure 1A to E) are seperated by tabs in the file.

1. Number of variables: 3 for each figure, 5 total

2. Number of cases/rows: 33-54 depending on the figure

3. Variable List:

* Days_of_isolation: # of days of isolation

* social_experience: type of social experience flies were exposed before testing (group or single housed & recovery)

* flies_within_4_body_lengths: # of flies within 4 body lengths for each treatment in the social space assay (average # of flies within 4 body lengths per chamber)

* sex: sex of the flies (male or female)

* aggregation index: sociability score or aggregation index for all treatments in the sociability assay (variance/mean # of flies in chamber)

1. Missing data codes: None

2. Specialized formats or other abbreviations used: GH: Group Housed, SH: Single Housed, REC: Recovery

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DATA-SPECIFIC INFORMATION FOR: Figure_2.xls

Specific graphs (Figure 2A to D) are seperated by tabs in the file.

1. Number of variables: 3 for Figure 2A & B, 2 for Figure 2C & D, 4 total

2. Number of cases/rows: 45-56 depending on the figure

3. Variable List:

* mating_status: mating status of flies used in behavioural experiments (either mated or virgin)

* social_experience: type of social experience flies were exposed before testing (group or single housed & recovery)

* flies_within_4_body_lengths: # of flies within 4 body lengths for each treatment in the social space assay (average # of flies within 4 body lengths per chamber)

* rearing_density: single housed (0) or # of flies group housed (2,6,16 & random uncontrolled number) prior to the social space assay (# of flies)

1. Missing data codes: None

2. Specialized formats or other abbreviations used: GH: Group Housed, SH: Single Housed, REC: Recovery

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DATA-SPECIFIC INFORMATION FOR: Figure_3.xls

Specific graphs (Figure 3A to D) are seperated by tabs in the file.

1. Number of variables: 3 for each figure, 3 total

2. Number of cases/rows: 36-41 depending on the figure

3. Variable List:

* genotype: genotype of flies used (either Cs [our control] or the nlg3Def1 mutant flies)

* social_experience: type of social experience flies were exposed before testing (group or single housed & recovery)

* flies_within_4_body_lengths: # of flies within 4 body lengths for each treatment in the social space assay (average # of flies within 4 body lengths per chamber)

1. Missing data codes: None

2. Specialized formats or other abbreviations used: GH: Group Housed, SH: Single Housed, REC: Recovery

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DATA-SPECIFIC INFORMATION FOR: Figure_4.xls

Specific graphs (Figure 4A to E) are seperated by tabs in the file.

1. Number of variables: 3 for Figure 4A to D, 6 total

2. Number of cases/rows: 35-49 depending on the figure

3. Variable List:

* genotype: genotype of flies used (either Cs [our control] or the nlg3Def1 mutant flies)

* social_experience: type of social experience flies were exposed before testing (group or single housed & recovery)

* flies_within_4_body_lengths: # of flies within 4 body lengths for each treatment in the social space assay (average # of flies within 4 body lengths per chamber)

* sex: sex of the flies (male or female)

* age: age of the flies during testing for dopamine levels (in days)

* dopamine_levels: dopamine levels in fly heads (picograms/head)

1. Missing data codes: None

2. Specialized formats or other abbreviations used: GH: Group Housed, SH: Single Housed, REC: Recovery

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DATA-SPECIFIC INFORMATION FOR: Figure_5.xls

Specific graphs (Figure 5A & B) are seperated by tabs in the file.

1. Number of variables: 3 for Figure 5A, 4 for Figure 5B

2. Number of cases/rows: 12 for Figure 5A, 11 for Figure 5B

3. Variable List:

* genotype: genotype of flies used (either Cs [our control] or the nlg3Def1 mutant flies)

* sex: sex of the flies (male or female)

* social_experience: type of social experience flies were exposed before testing (group or single housed & recovery)

* age: age of the flies during testing for dopamine levels (in days)

* dopamine_levels: dopamine levels in fly heads (picograms/head)

1. Missing data codes: None

2. Specialized formats or other abbreviations used: GH: Group Housed, SH: Single Housed, REC: Recovery

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DATA-SPECIFIC INFORMATION FOR: Supplemental_Figure_3.xls

Specific graphs (Supplemental Figure 3A & B) are seperated by tabs in the file.

1. Number of variables: 3

2. Number of cases/rows: 29-33

3. Variable List:

* genotype: genotype of flies used (either Cs [our control] or the nlg3Def1 mutant flies)

* social_experience: type of social experience flies were exposed before testing (group or single housed & recovery)

* flies_within_4_body_lengths: # of flies within 4 body lengths for each treatment in the social space assay (average # of flies within 4 body lengths per chamber)

1. Missing data codes: None

2. Specialized formats or other abbreviations used: GH: Group Housed, SH: Single Housed, REC: Recovery

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