Data from: Efficient in situ barcode sequencing using padlock probe-based BaristaSeq
Data files
Nov 21, 2018 version files 8.71 GB
-
data11.zip.001
943.72 MB
-
data11.zip.002
943.72 MB
-
data11.zip.003
354.72 MB
-
data12.zip.001
943.72 MB
-
data12.zip.002
943.72 MB
-
data12.zip.003
329.15 MB
-
data13.zip.001
943.72 MB
-
data13.zip.002
943.72 MB
-
data13.zip.003
163.74 MB
-
data14.zip.001
943.72 MB
-
data14.zip.002
943.72 MB
-
data14.zip.003
294.95 MB
-
script.zip
12.79 MB
Abstract
Cellular DNA/RNA tags (barcodes) allow for multiplexed cell lineage tracing and neuronal projection mapping with cellular resolution. Conventional approaches to reading out cellular barcodes trade off spatial resolution with throughput. Bulk sequencing achieves high throughput but sacrifices spatial resolution, whereas manual cell picking has low throughput. In situ sequencing could potentially achieve both high spatial resolution and high throughput, but current in situ sequencing techniques are inefficient at reading out cellular barcodes. Here we describe BaristaSeq, an optimization of a targeted, padlock probe-based technique for in situ barcode sequencing compatible with Illumina sequencing chemistry. BaristaSeq results in a five-fold increase in amplification efficiency, with a sequencing accuracy of at least 97%. BaristaSeq could be used for barcode-assisted lineage tracing, and to map long-range neuronal projections.