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Correlative 3D SBFSEM data from: Intermittent bulk release of human cytomegalovirus

Citation

Flomm, Felix et al. (2022), Correlative 3D SBFSEM data from: Intermittent bulk release of human cytomegalovirus, Dryad, Dataset, https://doi.org/10.5061/dryad.5dv41ns7z

Abstract

Human Cytomegalovirus (HCMV) can infect a variety of cell types by using virions of varying glycoprotein compositions. It is still unclear how this diversity is generated, but spatio-temporally separated envelopment and egress pathways might play a role. So far, one egress pathway has been described in which HCMV particles are individually enveloped into small vesicles and are subsequently exocytosed continuously. However, some studies have also found enveloped virus particles inside multivesicular structures but could not link them to productive egress or degradation pathways.
We used a novel 3D-CLEM workflow allowing us to investigate these structures in HCMV morphogenesis and egress at high spatio-temporal resolution. We found that multiple envelopment events occurred at individual vesicles leading to multiviral bodies (MViBs), which subsequently traversed the cytoplasm to release virions as intermittent bulk pulses at the plasma membrane to form extracellular virus accumulations (EVAs). Our data support the existence of a novel bona fide HCMV egress pathway, which opens the gate to evaluate divergent egress pathways in generating virion diversity.

Methods

Details on data acquisition and processing can be found in the original publication. The uploaded here are the unprocessed raw data.

Usage Notes

The data are in the proprietary dm4 format as saved by the acquisition software. However, they can be easily opened and converted in Fiji/ImageJ with the Bioformats importer. We recommend alignment of the stack and binning of 2x2 for working with the data.

Funding

Wellcome Trust, Award: 209250/Z/17/Z

Deutsche Forschungsgemeinschaft, Award: 390874280

Deutsche Forschungsgemeinschaft, Award: 413831413

Studienstiftung des Deutschen Volkes

Deutsche Forschungsgemeinschaft, Award: BO 4158/5-1

Freie und Hansestadt Hamburg, Award: LFF-FV 71-2019

Leibniz-Gemeinschaft, Award: W6/2018

Wellcome Trust, Award: 104913/Z/14/Z