Data from: The specific and exclusive microbiome of the deep-sea bone-eating snail, Rubyspira osteovora
Data files
Jan 04, 2017 version files 236.17 MB
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Bathym1-Int_seqs.fna
11.24 MB
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Bathym2-DG_seqs.fna
14.35 MB
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Phym751-int_seqs.fna
11.06 MB
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Ruby-PC41bone_seqs.fna
12.12 MB
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Ruby-PC44_seqs.fna
12.32 MB
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Ruby1162A-Int_seqs.fna
8.66 MB
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Ruby1162A-St_seqs.fna
8.87 MB
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Ruby204A-DG_seqs.fna
13.68 MB
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Ruby204A-St_seqs.fna
12.51 MB
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Ruby204B-St_seqs.fna
14.55 MB
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Ruby204C-DG_seqs.fna
13.48 MB
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Ruby204C-Fec_seqs.fna
15.60 MB
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Ruby204D-DG_seqs.fna
12.34 MB
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Ruby204D-Int_seqs.fna
11.22 MB
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Ruby769A-Int_seqs.fna
14.82 MB
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Ruby769A-St_seqs.fna
12.61 MB
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Ruby769B-Int_seqs.fna
17.43 MB
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Ruby769B-St_seqs.fna
16.81 MB
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Snail_data_OTUs_4_Dryad.xlsx
2.50 MB
Abstract
Rubyspira osteovora is an unusual deep-sea snail from Monterey Canyon, California. This group has only been found on decomposing whales and is thought to use bone as a novel source of nutrition. This study characterized the gut microbiome of R. osteovora, compared to the surrounding environment, as well as to other deep-sea snails with more typical diets. Analysis of 16S rRNA gene sequences revealed that R. osteovora digestive tissues host a much lower bacterial diversity (average Shannon index of 1.9; n = 12), compared to environmental samples (average Shannon index of 4.4; n = 2) and are dominated by two bacterial genera: Mycoplasma and Psychromonas (comprising up to 56% and 42% average total recovered sequences, respectively). These two bacteria, along with Psychrilyobacter sp. (∼16% average recovered sequences), accounted for between 43% and 92% of the total recovered sequences in individual snail digestive systems, with other OTUs present at much lower proportions. The relative abundance of these three groups remained similar over 6 years of sampling (collection date was not shown to be a significant predictor of community structure), suggesting a long-term association. Furthermore, these bacterial genera were either not present (Mycoplasma and Psychromonas) or at very low abundance (<0.04% for Psychrilyobacter), in environmental samples and other deep-sea gastropods, supporting the uniqueness of the R. osteovora gut microbiome.