Data from: Postmating prezygotic isolation occurs at two levels of divergence in Drosophila recens and D. subquinaria
Data files
Nov 27, 2024 version files 92.37 KB
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JEB-2023-00304_matings.csv
471 B
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JEB-2023-00304_SxR.csv
60.53 KB
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JEB-2023-00304_SxS.csv
26.89 KB
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README.md
4.48 KB
Abstract
Identifying the presence and strength of reproductive isolating barriers is necessary to understand how species form and then remain distinct in the face of ongoing gene flow. Here we study reproductive isolation at two stages of the speciation process in the closely related mushroom-feeding species Drosophila recens and D. subquinaria. We assess three isolating barriers that occur after mating, including the number of eggs laid, the proportion of eggs laid that hatched, and the number of adult offspring from a single mating. First, all three reproductive barriers are present between D. recensfemales and D. subquinaria males, which are at the late stages of speciation but still produce fertile daughters through which gene flow can occur. There is no evidence for geographic variation in any of these traits, concurrent with patterns of behavioral isolation. Second, all three of these reproductive barriers are strong between geographically distant conspecific populations of D. subquinaria, which are in the early stages of speciation and show genetic differentiation and asymmetric behavioral discrimination. The reduction in the number of eggs laid is asymmetric, consistent with patterns in behavioral isolation, and suggests the evolution of postmating prezygotic isolation due to cascade reinforcement against mating with D. recens. In summary, not only may postmating prezygotic reproductive barriers help maintain isolation between D. recens and D. subquinaria but they may also drive the earliest stages of isolation within D. subquinaria.
README: Data from: Postmating prezygotic isolation occurs at two levels of divergence in Drosophila recens and D. subquinaria
https://doi.org/10.5061/dryad.5hqbzkhfx
Authors: Duitsman, Bomar, Powell, Dyer.
Journal of Evolutionary Biology
Description of the data and file structure
Data include 1) behavioral assays of mating rate, copulation latency, and copulation duration; 2) number of eggs laid in 6 days, 3) proportion of eggs laid that hatched, 3) number of adult offspring produced after 20 days of egg laying. Data are included for crosses to test for 1) reproductive isolation between Drosophila recens females and D. subquinaria males and 2) between geographically distinct D. subquinaria populations.
Files and variables:
JEB-2023-00304_matings.csv
Experiment = Recens x Subquinaria or within subquinaria
Female = female species and population
Male = male species and population
Cross_FxM = Crosses are listed female x male
N_crosses = number of crosses set up
N_matings = number of crosses set up that mated
JEB-2023-00304_SxR.csv
Experiment: either fitness (offspring scored) or eggs produced/hatched
Fly_ID_dyrad: individual fly ID
Cross_FxM: Cross of female x male, noted as R_NY = recens NY, R_AB = recens alberta, S_MT = subquinaria montana,
Block: block
Copn Latency: copulation latency, time from male inserted to copulation started, noted in seconds. na = not recorded because not relevant to this particular cross. null = data not recorded even though it is part of the experiment.
Copn Duration: copulation duration, time from copulation start to end, noted in seconds. na = not recorded because not relevant to this particular cross. null = data not recorded even though it is part of the experiment.
Dead = the female died during the 6 or 20 days of the experiment or excaped during a transfer; number of offspring or eggs were not included. died = fly died, no = fly lived for the duration of the experiment.
Total offspring: number of offspring produced by the female across all days. null = no data usually because the fly died. na = no data because not relevant to this experiment.
total eggs hatched: total number of eggs hatched of those that were laid across all days. null = no data usually because the fly died. na = no data because not relevant to this experiment.
total eggs laid: total eggs laid across all days. null = no data usually because the fly died. na = no data because not relevant to this experiment.
Hatch day #, etc: number of eggs hatched in # egg laying vial. null = no data, either not relevant to this cross or fly died. na= no data because not relevant to the experiment.
Total day #, : number of eggs laid in # egg laying vial. null = no data usually because the fly died. na = no data because not relevant to this experiment.
JEB-2023-00304_SxS.csv
Experiment: either from the fitness experiment (offspring counted) or egg laying experiment (eggs scored)
Fly_ID_dyrad: individual fly ID
Cross_FxM: Cross of female x male, noted as OR = subquinaria oregon, AB = subquinaria alberta
Block: block
Copn Latency: copulation latency, time from male inserted to copulation started, noted in seconds. na = we did not record the time the male was placed in the vial or the beginning or end of copulation. na = not recorded because not relevant to this particular cross. null = data not recorded even though it is part of the experiment.
Copn Duration: copulation duration, time from copulation start to end, noted in seconds. na = we did not record the time the male was placed in the vial or the beginning or end of copulation. na = not recorded because not relevant to this particular cross. null = data not recorded even though it is part of the experiment.
Dead = the female died during the 6 or 20 days of the experiment or excaped during a transfer; number of offspring or eggs were not included. died = fly died, no = fly lived for the duration of the experiment.
Total offspring: number of offspring produced by the female across all days. null = no data usually because the fly died. na = no data because not relevant to this experiment.
total eggs hatched: total number of eggs hatched of those that were laid across all days
total eggs laid: total eggs laid across all days
Hatch day #, etc: number of eggs hatched in # egg laying vial
Total day #, : number of eggs laid in # egg laying vial
Methods
The data are the raw behavioral data and counts of eggs, their hatch rate, and number of adult offspring produced.