The distribution and absorption of ingested protein was characterized within a colony of Podocoryna carnea when a single polyp was fed. Observations were conducted at multiple spatial and temporal scales at three different stages of colony ontogeny with an artificial food item containing Texas Red conjugated albumin. Food pellets were digested and all tracer absorbed by digestive cells within the first 2–3 hours post-feeding. The preponderance of the label was located in the fed polyp and in a transport-induced diffusion pattern surrounding the fed polyp. After 6 hours post-feeding particulates re-appeared in the gastrovascular system and their absorption increased the area over which the nutrients were distributed, albeit still in a pattern that was centered on the fed polyp. At later intervals, tracer became concentrated in some stolon tips, but not in others, despite the proximity of these stolons either to the fed polyp or to adjacent stolons receiving nutrients. Distribution and absorption of nutrients is sequentially diffusive and directional.
B1_Replicate Early Ontogeny_Oct 1
Digitized images documenting colony morphology for replicate films. Scale bar = 1mm. Blue open circle corresponds to size and location of fed polyp. Red open circles correspond to size and locations of unfed stolons.
B2_Replicate Mid-Ontogeny_2 Jul
Digitized images documenting colony morphology for replicate films. Scale bar = 1mm. Blue open circle corresponds to size and location of fed polyp. Red open circles correspond to size and locations of unfed stolons.
B3 Replicate_Late Ontogeny_March 19
Digitized images documenting colony morphology for replicate films. Scale bar = 1mm. Blue open circle corresponds to size and location of fed polyp. Red open circles correspond to size and locations of unfed stolons.
B4_Replicate_Medusae-bearing Colony_8 July
Digitized images documenting colony morphology for replicate films. Scale bar = 1mm. Blue open circle corresponds to size and location of fed polyp. Red open circles correspond to size and locations of unfed stolons.
B5_Replicate Stolon Growth_20 Jun
Digitized images documenting colony morphology for replicate films. Scale bar = 1mm. Blue open circle corresponds to size and location of fed polyp. Red open circles correspond to size and locations of unfed stolons.
A1 Early ontogeny_15 Oct_Short
Early ontogeny colony_15 Oct_Short First image at 22 minutes post-feeding, subsequent images at 10 sec intervals. Exposure = 1 sec. The short exposure was used to quantify the luminosity of the fed polyp. Scale: 6.74 µm/pixel.
A2 Early ontogeny_15 Oct_Long
Early ontogeny colony_15 Oct_Long First image at 22 minutes post-feeding, subsequent images at 10 sec intervals. Exposure = 4 sec. The long exposure was used to quantify the luminosity of the unfed polyps and hydrorhiza. Scale: 6.74 µm/pixel.
A3 Mid-ontogeny colony_22 July
Mid-ontogeny colony_22 Jul First image at 43 minutes post-feeding, subsequent images at 3 hour intervals. Exposure = 5 sec. Scale: 2.4 µm/pixel.
A4 Late ontogeny_12 June_Short
Late ontogeny colony_12 June 2014_Short 1.3 First image at 16 minutes post-feeding, subsequent images at 10 sec intervals. Exposure = 0.5 sec. The short exposure was used to quantify the luminosity of the fed polyp. Scale: 5.97 µm/pixel.
A5 Late ontogeny_12 June_Long_pt 1
Late ontogeny colony_12 June 2014_Long First image at 16 minutes post-feeding, subsequent images at 10 sec intervals. Exposure = 5 sec. The short exposure was used to quantify the luminosity of the fed polyp. Scale: 5.97 µm/pixel. To meet upload specs, tiff files from this study have been divided into 2 folders- this is part 1.
A5 Late ontogeny_12 June_Long_pt 2
Late ontogeny colony_12 June 2014_Long_pt 2 First image at 16 minutes post-feeding, subsequent images at 10 sec intervals. Exposure = 5 sec. The short exposure was used to quantify the luminosity of the fed polyp. Scale: 5.97 µm/pixel. To meet upload specs, tiff files from this study have been divided into 2 folders- this is part 2.
A6 Medusa-bearing Colony_3 Oct_Short
Medusae-bearing colony_3 Oct_Short First image at 21 minutes post-feeding, subsequent images at 10 sec intervals. Exposure = 0.5 sec. The short exposure was used to quantify the luminosity of the fed polyp. Scale: 3.6 µm/pixel.
A7 Medusa-bearing Colony_3 Oct_Long_pt 1
Medusae-bearing colony_3 Oct_Long_pt 1 First image at 21 minutes post-feeding, subsequent images at 10 sec intervals. Exposure = 2 sec. The long exposure was used to quantify the luminosity of the unfed polyps and hydrorhiza. Scale: 3.6 µm/pixel.
To meet upload specs, tiff files from this study have been divided into 2 folders- this is part 1.
A7 Medusa-bearing Colony_3 Oct_Long_pt 2
Medusae-bearing colony_3 Oct_Long_pt 2 First image at 21 minutes post-feeding, subsequent images at 10 sec intervals. Exposure = 2 sec. The long exposure was used to quantify the luminosity of the unfed polyps and hydrorhiza. Scale: 3.6 µm/pixel.
To meet upload specs, tiff files from this study have been divided into 2 folders- this is part 2.
A8 Cut Stolon study_1 March
Cut stolon experiment_1 Mar First image at 35 minutes post-feeding, subsequent images at 1 hour intervals. Exposure for frames 1-5 = 4 sec, exposure for remaining frames = 8 sec. Scale: 3.26 µm/pixel.
A9 Fed Polyp_10 Sept
Fed Polyp_10 Sept First image at 15 minutes post-feeding, subsequent images at 10 second intervals. Exposure = 4 sec. Scale: 3.08 µm/pixel.
C1 Replicate Early ontogeny_1 Oct_Short
Replicate_Early Ontogeny_1 Oct_Short First image at 25 minutes post-feeding, subsequent images at 10 second intervals. Exposure = 0.5 sec. Scale: 6.74 µm/pixel. The short exposure may be used to quantify the luminosity of the fed polyp.
C2 Replicate_Early ontogeny_1 Oct_Long
Replicate_Early Ontogeny_1 Oct_ Long First image at 25 minutes post-feeding, subsequent images at 10 second intervals. Exposure = 2 sec. Scale: 6.74 µm/pixel. The long exposure may be used to quantify the luminosity of unfed polyps and hydrorhiza.
C3 Replicate_Mid Ontogeny_2 July_short
Replicate_Mid-Ontogeny_2 Jul_Short First image at 24 minutes post-feeding, subsequent images at 9 second intervals. Exposure = 0.5 sec. Scale: 6.74 µm/pixel. The short exposure may be used to quantify the luminosity of the fed polyp.
C4 Replicate_Mid Ontogeny_2 July_long
Replicate_Mid-Ontogeny_2 Jul_Long First image at 24 minutes post-feeding, subsequent images at 9 second intervals. Exposure = 5 sec. Scale: 6.74 µm/pixel. The long exposure may be used to quantify the luminosity of unfed polyps and hydrorhiza.
C5 Replicate_Late ontogeny_19 March_Short
Replicate_Late Ontogeny_19 Mar_Short First image at 35 minutes post-feeding, subsequent images at 9 second intervals. Exposure = 50 msec. Scale: 6.74 µm/pixel. The short exposure may be used to quantify the luminosity of the fed polyp.
C6 Replicate_Late ontogeny_19 March_Long_pt 1
Replicate_Late Ontogeny_19 Mar_Long_pt 1 First image at 35 minutes post-feeding, subsequent images at 9 second intervals. Exposure = 5 sec. Scale: 6.74 µm/pixel. The long exposure may be used to quantify the luminosity of unfed polyps and hydrorhiza. To meet upload specs, tiff files from this study have been divided into 2 folders- this is part 1.
C6 Replicate_Late ontogeny_19 March_Long_pt 2
Replicate_Late Ontogeny_19 Mar_Long_pt 2 First image at 35 minutes post-feeding, subsequent images at 9 second intervals. Exposure = 5 sec. Scale: 6.74 µm/pixel. The long exposure may be used to quantify the luminosity of unfed polyps and hydrorhiza. To meet upload specs, tiff files from this study have been divided into 2 folders- this is part 2.
C7 Replicate_Medusa-bearing_8 July_Short
Replicate_Medusae-bearing_ 8 Jul_Short First image at 19 minutes post-feeding, subsequent images at 9 second intervals. Exposure = 0.5 sec. Scale: 6.74 µm/pixel. The short exposure may be used to quantify the luminosity of the fed polyp.
C8 Replicate_Medusa-bearing_8 July_Long_pt 1
Replicate_Medusae-bearing_ 8 Jul_Long_part 1 First image at 19 minutes post-feeding, subsequent images at 9 second intervals. Exposure = 5 sec. Scale: 6.74 µm/pixel. The long exposure may be used to quantify the luminosity of unfed polyps and hydrorhiza. To meet upload specs, tiff files from this study have been divided into 2 folders- this is part 1.
C8 Replicate_Medusa-bearing_8 July_Long_pt 2
Replicate_Medusae-bearing_ 8 Jul_Long_part 2 First image at 19 minutes post-feeding, subsequent images at 9 second intervals. Exposure = 5 sec. Scale: 6.74 µm/pixel. The long exposure may be used to quantify the luminosity of unfed polyps and hydrorhiza. To meet upload specs, tiff files from this study have been divided into 2 folders- this is part 2.
C9 Replicate_Stolon Growth_20 June
Replicate_ Stolon Growth_20 Jun First image at 27 minutes post-feeding, subsequent images at 3 hour intervals. Exposure = 0.45 sec for frames 1-8, 4 sec for frames 9-24. sec. Scale: 2.45 µm/pixel.
C10 Replicate_Fed Polyp_17 Sept
Replicate_Fed Polyp_17 Sept First image at 20 minutes post-feeding, subsequent images at 10 second intervals. Exposure = 0.75 sec. Scale: 3.27 µm/pixel.