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Imaging dataset for positron emission microscopy of patient-derived tumor organoids

Citation

Khan, Syamantak; Pratx, Guillem (2021), Imaging dataset for positron emission microscopy of patient-derived tumor organoids, Dryad, Dataset, https://doi.org/10.5061/dryad.63xsj3v2v

Abstract

Here, we present a microscopy method to image 18F-fluorodeoxyglucose and other radiotracers in patient-derived tumor organoids with spatial resolution up to 100-fold better than that of clinical positron emission tomography (PET). When combined with brightfield imaging, this metabolic imaging approach functionally mirrors clinical PET/CT scans and provides a quantitative readout of cell glycolysis other metabolic processes. 

Methods

The dataset was collected in a custom-built radioluminescence microscope setup. The images are collected by an EMCCD (Hamamatsu). The images are raw and unprocessed. 

Usage Notes

Each image file is accompanied by a metafile that documents all the settings used for EMCCD acquisition. The name of the folders describes the experiment or specific modality used for imaging. The readme file summarizes the abbreviations used in the folder names.