The success of microbial termiticides in controlling termites depends on the ability of microbes to grow in different medium and the functionality of the microbes as a resistant barrier or toxic bait. This study was conducted to understand the mortality rate and behaviour changes of subterranean termite (Coptotermes curvignathus Holmgren) introduced with different concentrations of Serratia marcescens strain LGMS 1 and Pseudomonas aeruginosa strain LGMS 3 using wood and soil as bacterial transfer medium. In general, higher concentration of bacteria in soil caused reduction in tunnelling activity, wood consumption and an increased in mortality. However, application on wood revealed a different outcome. Wood treated with S. marcescens of 10⁶ CFU/mL concentration proved to be more efficient as bait than higher concentration applications as it caused high mortality rate while still highly palatable for termites. Wood or soil treated with S. marcescens concentration higher than 10⁹ CFU/mL creates a high toxicity and repellent barrier for termites. P. aeruginosa of 10⁹ CFU/mL concentrations applied on wood served as a slow-acting toxic bait. However, the ability for S. marcescens and P. aeruginosa to survive on wood are low which caused the bait unable to retain a useful level of toxicity for a long period of time and frequent reapplication is needed.