Data from: Whole-chloroplast analysis as an approach for fine-tuning the preservation of a highly charismatic but critically endangered species, Wollemia nobilis (Araucariaceae)
Greenfield, Abigail, UNSW Sydney
McPherson, Hannah, Royal Botanic Garden Sydney
Auld, Tony, NSW Office of Environment & Heritage
Delaney, Sven, UNSW Sydney
Offord, Catherine A., The Australian PlantBank, Australian Botanic Garden, Narellan Road, Mount Annan, NSW, 2567, Australia.
van der Merwe, Marlien, Royal Botanic Garden Sydney
Yap, Jia-Yee S., Royal Botanic Garden Sydney
Rossetto, Maurizio, Royal Botanic Garden Sydney
Published Oct 12, 2017 on Dryad.
Cite this dataset
Greenfield, Abigail et al. (2017). Data from: Whole-chloroplast analysis as an approach for fine-tuning the preservation of a highly charismatic but critically endangered species, Wollemia nobilis (Araucariaceae) [Dataset]. Dryad. https://doi.org/10.5061/dryad.6908r
The critically endangered Wollemia nobilis W.G. Jones, K.D. Hill & J.M. Allen is endemic to Wollemi National Park north of Sydney (Australia). All known wild individuals are restricted to four sites in a single canyon system. W. nobilis can reproduce sexually but at all sites individual clumps can be multi-stemmed from a common base. In the first genetic study of this species, no genetic variation was found across multiple genetic marker types representing hundreds of nuclear loci, indicating this species is characterised by very low genetic variation. In this study we searched for variation across the chloroplast using shotgun sequencing, bioinformatic extraction of chloroplast DNA and variant detection. Six chloroplast single nucleotide polymorphisms were detected, producing three chlorotypes. Chlorotype 1 is found in every individual surveyed at Sites 1 and 3, and in individuals from Sites 2 and 4. Chlorotype 2 – the most distinct chlorotype – was found in two individuals from Site 4. Chlorotype 3 consists of a single difference from Chlorotype 1 and may represent a somaclonal mutant. These findings will guide management and translocation of this critically endangered species. This study provides a practical template that is highly informative and easily applicable to other taxa in similar circumstances.
Wollemia nobilis chloroplast primers and sequences
The data file contains primer sequences designed to verify putative SNPs in the chloroplast DNA of Wollemia nobilis. The sequences that contributed to each of the three chlorotypes characterised in the paper are included along with the position of the variant in the sequence (where one was detected).