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Genetic analysis of SnRK1β3 subunit of peach and the functional identification of overexpression transformed tomato

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Aug 30, 2024 version files 3.07 MB
Sep 23, 2024 version files 3.07 MB
Nov 08, 2024 version files 3.07 MB

Abstract

The sucrose non-fermentation-related kinase 1 (SnRK1) protein complex in plants plays an important role in energy metabolism, anabolism, growth, and stress resistance. This protein complex typically consists of an α subunit, three β subunits, one or two βγ subunits, and a γ subunit. Studies on plant SnRK1 have primarily focused on the functional α subunit, with the β regulatory subunit remaining relatively unexplored. The present study aimed to elucidate the evolutionary relationship, structural prediction, and interaction with the core α subunit of peach SnRK1β3 (PpSnRK1) subunit. In addition, we produced transgenic tomato plants overexpressing PpSnRK1 (OEPpSnRK1). We mainly tested the growth index and drought resistance of transgenic tomato plants.The results showed that PpSnRK1 has a 354 bp encoded protein sequence (cds), which is mainly located in the nucleus and cytoplasm. Phylogenetic tree analysis showed that PpSnRK1β3 has similar domains to other woody plants. Transcriptome analysis of OEPpSnRK1β3 showed that PpSnRK1β3 is widely involved in plant physiological regulation processes and stress response. Functional analyses of these transgenic plants revealed prolonged growth periods, enhanced growth potential, improved photosynthetic activity and superior drought stress tolerance.