Genetic analysis of SnRK1β3 subunit of peach and the functional identification of overexpression transformed tomato
Data files
Aug 30, 2024 version files 3.07 MB
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a-BD-1.ab1
315.78 KB
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a-BD-2.ab1
308.47 KB
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a-bifc-1.ab1
304 KB
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a-bifc-2.ab1
306.36 KB
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a-nluc-1.ab1
304.92 KB
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a-nluc-2.ab1
315.82 KB
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b3-AD.ab1
307.41 KB
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b3-bifc.ab1
294.46 KB
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b3-cluc.ab1
292.82 KB
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b3-GFP.ab1
307.41 KB
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CDS-α-DNA.fa
1.61 KB
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CDS-β3-DNA.fa
372 B
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Evolutionary_tree_proteins.fa
1.83 KB
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Physiological_data.txt
2.14 KB
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README.md
3.72 KB
Sep 23, 2024 version files 3.07 MB
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a-BD-1.ab1
315.78 KB
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a-BD-2.ab1
308.47 KB
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a-bifc-1.ab1
304 KB
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a-bifc-2.ab1
306.36 KB
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a-nluc-1.ab1
304.92 KB
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a-nluc-2.ab1
315.82 KB
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b3-AD.ab1
307.41 KB
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b3-bifc.ab1
294.46 KB
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b3-cluc.ab1
292.82 KB
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b3-GFP.ab1
307.41 KB
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CDS-α-DNA.fa
1.61 KB
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CDS-β3-DNA.fa
372 B
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Evolutionary_tree_proteins.fa
1.83 KB
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Physiological_data.txt
2.14 KB
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README.md
3.72 KB
Nov 08, 2024 version files 3.07 MB
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a-BD-1.ab1
315.78 KB
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a-BD-2.ab1
308.47 KB
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a-bifc-1.ab1
304 KB
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a-bifc-2.ab1
306.36 KB
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a-nluc-1.ab1
304.92 KB
-
a-nluc-2.ab1
315.82 KB
-
b3-AD.ab1
307.41 KB
-
b3-bifc.ab1
294.46 KB
-
b3-cluc.ab1
292.82 KB
-
b3-GFP.ab1
307.41 KB
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CDS-α-DNA.fa
1.61 KB
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CDS-β3-DNA.fa
372 B
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Evolutionary_tree_proteins.fa
1.83 KB
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Physiological_data.txt
2.14 KB
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README.md
4.34 KB
Abstract
The sucrose non-fermentation-related kinase 1 (SnRK1) protein complex in plants plays an important role in energy metabolism, anabolism, growth, and stress resistance. This protein complex typically consists of an α subunit, three β subunits, one or two βγ subunits, and a γ subunit. Studies on plant SnRK1 have primarily focused on the functional α subunit, with the β regulatory subunit remaining relatively unexplored. The present study aimed to elucidate the evolutionary relationship, structural prediction, and interaction with the core α subunit of peach SnRK1β3 (PpSnRK1) subunit. In addition, we produced transgenic tomato plants overexpressing PpSnRK1 (OEPpSnRK1). We mainly tested the growth index and drought resistance of transgenic tomato plants.The results showed that PpSnRK1 has a 354 bp encoded protein sequence (cds), which is mainly located in the nucleus and cytoplasm. Phylogenetic tree analysis showed that PpSnRK1β3 has similar domains to other woody plants. Transcriptome analysis of OEPpSnRK1β3 showed that PpSnRK1β3 is widely involved in plant physiological regulation processes and stress response. Functional analyses of these transgenic plants revealed prolonged growth periods, enhanced growth potential, improved photosynthetic activity and superior drought stress tolerance.
README: Genetic analysis of SnRK13 subunit of peach and the functional identification of overexpression transformed tomato
https://doi.org/10.5061/dryad.6djh9w19t
Description of the data and file structure
The files uploaded for this assay include sequencing files (b3-bifc.ab1, a-bifc-2.ab1, a-nluc-1.ab1, b3-cluc.ab1, a-nluc-2.ab1, a-bifc-1.ab1, a-BD-1.ab1, a-BD-2.ab1, b3-AD.ab1,b3-GFP.ab1), sequence files (CDS-α-DNA.fa, CDS-β3-DNA.fa, Evolutionary tree proteins.fa) and phenotypic raw data file (Physiological data.txt).
b3-bifc.ab1, a-bifc-2.ab1,a-bifc-1.ab1
Sequencing results of PpSnRK1α and PpSnRKβ3 interaction construction vectors identified by Bifc assay (sequence + peak plots).
a-nluc-1.ab1,a-nluc-2.ab1,b3-cluc.ab1
Sequencing results of PpSnRK1α and PpSnRKβ3 interaction construction vectors identified by Dual luciferase assay (sequence + peak plots).
a-BD-1.ab1, a-BD-2.ab1,b3-AD.ab1
Sequencing results of PpSnRK1α and PpSnRKβ3 interaction construction vectors identified by Yeast two-hybrid assay (sequence + peak plots).
b3-GFP.ab1
Sequencing results of the overexpression vector PpSnRKβ3-GFP (sequence + peak plots).
CDS-α-DNA.fa
PpSnRK1α(Prupe.3G262900) cds from the NCBI database (https://www.ncbi.nlm.nih.gov).
CDS-β3-DNA.fa
PpSnRK1β3 (Prupe.6G107300) cds from the NCBI database (https://www.ncbi.nlm.nih.gov).
Evolutionary tree proteins.fa
For phylogenetic tree construction, we selected PpSnRK1β3 along with several other plant-encoded proteins:AtSnRK1β3(NP_001323590.1),PtSnRK1β3(XP_006384933.1),PpSnRK1β3(XP_006384933.1),VvSnRK1β3(XP_019076263.1),MdSnRK1β3(XP_008359553.1),PaSnRK1β3(XP_021812278.1),AcSnRK1β3(XP_020085356.1),NaSnRK1β3(XP_019255734.1),CiSnRK1β3(XP_042955180.1),MiSnRK1β3(XP_044506124.1),DzSnRK1β3(XP_022732265.1),PbSnRK1β3(XP_048444836.1),TaSnRK1β3(XP_044447578.1),GhSnRK1β3(XP_016748682.2) from the NCBI database (https://www.ncbi.nlm.nih.gov).
Physiological data.txt
The role of PpSnRK1β3 in tomato plants was explored by introducing the coding sequences seqences that encode protein into stem segments infected with the Pri-101 vector. We compared the phenotypic differences of overexpressing PpSnRK1β3 (OEPpSnRK1β3) with WT tomatoes.
Comparison of plant height, stem diameter, leaf area, number of days in the growth period for three strains of OEPpSnRK1β3 and WT tomatoes.
Comparison of maximum net photosynthetic efficiency, chlorophyll content, stomatal conductance , intercellular carbon dioxide concentration for three strains of OEPpSnRK1β3 and WT tomatoes.
The state of OEPpSnRK1β3 and WT tomatoes under normal and 14-day drought stress. Comparison of maximum photochemical efficiency , malondialdehyde content , hydrogen peroxide content, superoxide anion content , relative electrolyte leakage for three strains of OEPpSnRK1β3 and WT tomatoes under normal and 14-day drought stress conditions.
Software
Sequencing data in ab1 format can be downloaded for viewing on Snapgene Software (https://www.snapgene.com/).Import the target sequences queried on the NCBI database, and then select aligned sequences in the toolbar to import the sequencing data, and the two can be compared.
Version changes
23-Sep**-2024 **The phenotypic data H2O2 content and O2- content data were uploaded incorrectly,the data was re-uploaded.
**10-Nov-2024 **Phenotypic data: Due to the phenotypic identification of some of the same indicators at the same time in our different transgenic materials, some data were uploaded incorrectly. Through identification, we updated the plant height, stem base width, and SPAD value data of overexpressed tomatoes.The chlorophyll content data uploaded in the previous version is actually chlorophyll a content, and the total chlorophyll content data has been updated.
Access information
Other publicly accessible locations of the data:
- None
Data was derived from the following sources:
- Genetic analysis of SnRK13 subunit of peach and the functional identification of overexpression transformed tomato.