GENERAL INFORMATION

1. Title of Dataset: Functions of the Sulfatase-Modifying Factor 1 (HaSumf1) in the development and host glucosinolates adaptation of Helicoverpa armigera Access this dataset on Dryad
2. Author Information A. Principal Investigator Contact Information Name: Long Zhang Institution: Xinjiang University Email: lonzhang@foxmail.com B. Associate or Co-investigator Contact Information Name: Zhiran Zhou Institution: Xinjiang University Name: Fan Cai Institution: Xinjiang University Name: Wanli Qin Institution: Xinjiang University Name: Li Liu Institution: Xinjiang University Name: Guli Kuerban Institution: Xinjiang University Name: Lin Jin Institution: Nanjing Agricultural University Name: Xiaoli Ma Institution: Xinjiang University
3. Information about funding sources: National Natural Science Foundation of China, Award: 32060620; Natural Science Foundation of the Autonomous Region, Award: 2020D01C060

SHARING/ACCESS INFORMATION

1. Licenses/restrictions placed on the data: CC0 1.0 Universal (CC0 1.0) Public Domain
2. Links to publications that cite or use the data: Zhang, L., Zhou, Z., Cai, F., Qin, W., Liu, L., Kuerban, G., Jin, L., Ma, X. (2023). Functions of the Sulfatase-Modifying Factor 1 (HaSumf1) in the development and host glucosinolates adaptation of Helicoverpa armigera. Journal of Applied Entomology.
3. Links to other publicly accessible locations of the data: None
4. Links/relationships to ancillary data sets: None
5. Was data derived from another source? No A. If yes, list source(s): NA
6. Recommended citation for this dataset: Zhang, L., Zhou, Z., Cai, F., Qin, W., Liu, L., Kuerban, G., Jin, L., Ma, X. (2023). Data from: Functions of the Sulfatase-Modifying Factor 1 (HaSumf1) in the development and host glucosinolates adaptation of Helicoverpa armigera. Dryad Digital Repository. https://doi.org/10.5061/dryad.6q573n64w

DATA & FILE OVERVIEW
In this study, we detailed the indicators of development of H. armigera, such as growth and development period from first- to fifth-instar, number and weight of surviving larvae at fifth-instar and pupa, as well as the relative expression levels of HaSumf1 gene at different instars and the third- and fifth-instars after feeding with glucosinolates and/or dsSumf1.

1. File List: A) Expression of HaSumf1 at different instars.csv B) Expression of HaSumf1 at third-instar.csv C) Expression of HaSumf1 at fifth-instar.csv D) Growth and development period of larvae.csv E) Number of surviving larvae.csv F) Number of surviving pupa.csv G) Weight of surviving larvae.csv H) Weight of surviving pupa.csv
2. Relationship between files, if important: None
3. Additional related data collected that was not included in the current data package: None
4. Are there multiple versions of the dataset? No A. If yes, name of file(s) that was updated: NA i. Why was the file updated? NA ii. When was the file updated? NA

DATA-SPECIFIC INFORMATION FOR: Expression of HaSumf1 at different instars.csv

1. Number of variables: 9
2. Number of cases/rows: 27
3. Variable List:
   • Instars: First-instar, Second-instar, Third-instar, Fourth-instar, Fifth-instar, Prepupa, Pupa, Male adult, Female adult
   • CT (HaSumf1): Threshold cycle (CT) of HaSumf1 measured by qRT-PCR
   • CT (RPS15): Threshold cycle (CT) of RPS15 ( internal control genes) measured by qRT-PCR
   • CT_average (RPS15): Average value of threshold cycle (CT) of RPS15 ( internal control genes) measured by qRT-PCR
   • Delta_CT=CT(HaSumf1)-CT_average (RPS15): D-value between CT (HaSumf1) and CT (RPS15)
   • Delta_CT (average): Average value of D-value between CT (HaSumf1) and CT (RPS15)
   • 2 Delta_CT=Delta_CT(HaSumf1)-Delta_CT(RPS15): D-value between Delta_CT(HaSumf1) and Delta_CT(average)
   • 2^(-2 Delta_CT): Relative expression level of HaSumf1 gene
   • Average: Average value of relative expression level of HaSumf1 gene
4. Missing data codes: 'n/a'
5. Specialized formats or other abbreviations used: None

DATA-SPECIFIC INFORMATION FOR: Expression of HaSumf1 at third-instar.csv

1. Number of variables: 11
2. Number of cases/rows: 27
3. Variable List:
   • Groups: Control (feeding normal diet and pure water), dsEGFP (feeding normal diet and dsEGFP), dsSumf1 (feeding normal diet and dsSumf1), 0.004%GLS (feeding diet containing 0.004%GLS and pure water), 0.004%GLS+dsEGFP (feeding diet containing 0.004%GLS and dsEGFP), 0.004%GLS+dsSumf1 (feeding diet containing 0.004%GLS and dsSumf1), 0.04%GLS (feeding diet containing 0.04%GLS and pure water), 0.04%GLS+dsEGFP (feeding diet containing 0.04%GLS and dsEGFP), 0.04%GLS+dsSumf1 (feeding diet containing 0.004%GLS and dsSumf1)
   • CT (HaSumf1): Threshold cycle (CT) of HaSumf1 measured by qRT-PCR
   • CT (RPS15): Threshold cycle (CT) of RPS15 ( internal control genes) measured by qRT-PCR
   • CT_average (RPS15): Average value of threshold cycle (CT) of RPS15 ( internal control genes) measured by qRT-PCR
   • Delta_CT=CT(HaSumf1)-CT_average (RPS15): D-value between CT (HaSumf1) and CT (RPS15)
   • Delta_CT (average): Average value of D-value between CT (HaSumf1) and CT (RPS15)
   • 2 Delta_CT=Delta_CT(HaSumf1)-Delta_CT(RPS15): D-value between Delta_CT(HaSumf1) and Delta_CT(average)
   • 2^(-2 Delta_CT): Relative expression level of HaSumf1 gene
   • Average: Average value of relative expression level of HaSumf1 gene
   • Treatment-Control: Changes in the treatment group compared to the control group
   • (Treatment-Control)/Control: The ratio of changes in the treatment group compared to the control group
4. Missing data codes: 'n/a'
5. Specialized formats or other abbreviations used: None

DATA-SPECIFIC INFORMATION FOR: Expression of HaSumf1 at fifth-instar.csv

1. Number of variables: 11
2. Number of cases/rows: 27
3. Variable List:
   • Groups: Control (feeding normal diet and pure water), dsEGFP (feeding normal diet and dsEGFP), dsSumf1 (feeding normal diet and dsSumf1), 0.004%GLS (feeding diet containing 0.004%GLS and pure water), 0.004%GLS+dsEGFP (feeding diet containing 0.004%GLS and dsEGFP), 0.004%GLS+dsSumf1 (feeding diet containing 0.004%GLS and dsSumf1), 0.04%GLS (feeding diet containing 0.04%GLS and pure water), 0.04%GLS+dsEGFP (feeding diet containing 0.04%GLS and dsEGFP), 0.04%GLS+dsSumf1 (feeding diet containing 0.004%GLS and dsSumf1)
   • CT (HaSumf1): Threshold cycle (CT) of HaSumf1 measured by qRT-PCR
   • CT (RPS15): Threshold cycle (CT) of RPS15 (internal control genes) measured by qRT-PCR
   • CT_average (RPS15): Average value of threshold cycle (CT) of RPS15 (internal control genes) measured by qRT-PCR
   • Delta_CT=CT(HaSumf1)-CT_average (RPS15): D-value between CT (HaSumf1) and CT (RPS15)
   • Delta_CT (average): Average value of D-value between CT (HaSumf1) and CT (RPS15)
   • 2 Delta_CT=Delta_CT(HaSumf1)-Delta_CT(RPS15): D-value between Delta_CT(HaSumf1) and Delta_CT(average)
   • 2^(-2 Delta_CT): Relative expression level of HaSumf1 gene
   • Average: Average value of relative expression level of HaSumf1 gene
   • Treatment-Control: Changes in the treatment group compared to the control group
   • (Treatment-Control)/Control: The ratio of changes in the treatment group compared to the control group
4. Missing data codes: 'n/a'
5. Specialized formats or other abbreviations used: None

DATA-SPECIFIC INFORMATION FOR: Growth and development period of larvae.csv

1. Number of variables: 8
2. Number of cases/rows: 495
3. Variable List:
   • Treatment: Control (feeding normal diet and pure water), dsEGFP (feeding normal diet and dsEGFP), dsSumf1 (feeding normal diet and dsSumf1), 0.004%GLS (feeding diet containing 0.004%GLS and pure water), 0.004%GLS+dsEGFP (feeding diet containing 0.004%GLS and dsEGFP), 0.004%GLS+dsSumf1 (feeding diet containing 0.004%GLS and dsSumf1), 0.04%GLS (feeding diet containing 0.04%GLS and pure water), 0.04%GLS+dsEGFP (feeding diet containing 0.04%GLS and dsEGFP), 0.04%GLS+dsSumf1 (feeding diet containing 0.004%GLS and dsSumf1)
   • Groups: Each Treatment is divided into 3 groups A (including 18 samples), B (including 18 samples), and C (including 19 samples)
   • Number: Number of each sample
   • First_instar: The period from hatching of the first larva to individual rearing of each larva is 2 days
   • Second_instar: The period from individual rearing of each larva to molting at second-instar, in days
   • Third_instar: The period from molting at second-instar to molting at third-instar, in days
   • Fourth_instar: The period from molting at third-instar to molting at fourth-instar, in days
   • Fifth_instar: The period from molting at fourth-instar to molting at fifth-instar, in days
4. Missing data codes: 'n/a' represents the death sample
5. Specialized formats or other abbreviations used: The developmental duration of larvae (the duration of the entire larval stage from first- to fifth-instar)

DATA-SPECIFIC INFORMATION FOR: Weight of surviving larvae.csv

1. Number of variables: 4
2. Number of cases/rows: 495
3. Variable List:
   • Treatment: Control (feeding normal diet and pure water), dsEGFP (feeding normal diet and dsEGFP), dsSumf1 (feeding normal diet and dsSumf1), 0.004%GLS (feeding diet containing 0.004%GLS and pure water), 0.004%GLS+dsEGFP (feeding diet containing 0.004%GLS and dsEGFP), 0.004%GLS+dsSumf1 (feeding diet containing 0.004%GLS and dsSumf1), 0.04%GLS (feeding diet containing 0.04%GLS and pure water), 0.04%GLS+dsEGFP (feeding diet containing 0.04%GLS and dsEGFP), 0.04%GLS+dsSumf1 (feeding diet containing 0.004%GLS and dsSumf1)
   • Groups: Each Treatment is divided into 3 groups A (including 18 samples), B (including 18 samples), and C (including 19 samples)
   • Number: Number of each sample
   • Body weight (mg): The larval body weight measurements within 24-36 hours of molting at fifth-instar
4. Missing data codes: 'n/a' represents the death sample
5. Specialized formats or other abbreviations used: None

DATA-SPECIFIC INFORMATION FOR: Weight of surviving pupa.csv

1. Number of variables: 4
2. Number of cases/rows: 495
3. Variable List:
   • Treatment: Control (feeding normal diet and pure water), dsEGFP (feeding normal diet and dsEGFP), dsSumf1 (feeding normal diet and dsSumf1), 0.004%GLS (feeding diet containing 0.004%GLS and pure water), 0.004%GLS+dsEGFP (feeding diet containing 0.004%GLS and dsEGFP), 0.004%GLS+dsSumf1 (feeding diet containing 0.004%GLS and dsSumf1), 0.04%GLS (feeding diet containing 0.04%GLS and pure water), 0.04%GLS+dsEGFP (feeding diet containing 0.04%GLS and dsEGFP), 0.04%GLS+dsSumf1 (feeding diet containing 0.004%GLS and dsSumf1)
   • Groups: Each Treatment is divided into 3 groups A (including 18 samples), B (including 18 samples), and C (including 19 samples)
   • Number: Number of each sample
   • Pupa weight (mg): The pupa weight measurements within 24-36 hours of pupation
4. Missing data codes: 'n/a' represents the death sample
5. Specialized formats or other abbreviations used: None

DATA-SPECIFIC INFORMATION FOR: Number of surviving larvae.csv

1. Number of variables: 4
2. Number of cases/rows: 27
3. Variable List:
   • Treatment: Control (feeding normal diet and pure water), dsEGFP (feeding normal diet and dsEGFP), dsSumf1 (feeding normal diet and dsSumf1), 0.004%GLS (feeding diet containing 0.004%GLS and pure water), 0.004%GLS+dsEGFP (feeding diet containing 0.004%GLS and dsEGFP), 0.004%GLS+dsSumf1 (feeding diet containing 0.004%GLS and dsSumf1), 0.04%GLS (feeding diet containing 0.04%GLS and pure water), 0.04%GLS+dsEGFP (feeding diet containing 0.04%GLS and dsEGFP), 0.04%GLS+dsSumf1 (feeding diet containing 0.004%GLS and dsSumf1)
   • Groups: Each Treatment is divided into 3 groups A (including 18 samples), B (including 18 samples), and C (including 19 samples)
   • Initial number of larvae: The number of larvae used for testing in groups A, B and C
   • Number of surviving larvae: Number of surviving larvae when measuring body weight at fifth-instar
4. Missing data codes: None
5. Specialized formats or other abbreviations used: The larval survival rate (the ratio of the number of surviving at fifth-instar to the initial number of larvae)

DATA-SPECIFIC INFORMATION FOR: Number of surviving pupa.csv

1. Number of variables: 4
2. Number of cases/rows: 27
3. Variable List:
   • Treatment: Control (feeding normal diet and pure water), dsEGFP (feeding normal diet and dsEGFP), dsSumf1 (feeding normal diet and dsSumf1), 0.004%GLS (feeding diet containing 0.004%GLS and pure water), 0.004%GLS+dsEGFP (feeding diet containing 0.004%GLS and dsEGFP), 0.004%GLS+dsSumf1 (feeding diet containing 0.004%GLS and dsSumf1), 0.04%GLS (feeding diet containing 0.04%GLS and pure water), 0.04%GLS+dsEGFP (feeding diet containing 0.04%GLS and dsEGFP), 0.04%GLS+dsSumf1 (feeding diet containing 0.004%GLS and dsSumf1)
   • Groups: Each Treatment is divided into 3 groups A (including 18 samples), B (including 18 samples), and C (including 19 samples)
   • Number of surviving larvae: Number of surviving larvae when measuring body weight at fifth-instar
   • Number of surviving pupa: Number of surviving pupa when measuring weight of pupa
4. Missing data codes: None
5. Specialized formats or other abbreviations used: The pupation rate (the ratio of the number of pupa to surviving larvae at fifth-instar)