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Mitochondrial genomes and thousands of ultraconserved elements resolve the taxonomy and historical biogeography of the Euphonia and Chlorophonia finches (Passeriformes: Fringillidae)

Citation

Imfeld, Tyler; Barker, Keith; Brumfield, Robb (2021), Mitochondrial genomes and thousands of ultraconserved elements resolve the taxonomy and historical biogeography of the Euphonia and Chlorophonia finches (Passeriformes: Fringillidae), Dryad, Dataset, https://doi.org/10.5061/dryad.6t1g1jwvv

Abstract

Relationships of the Neotropical finches in the genera Euphonia and Chlorophonia (Fringillidae: Euphoniinae) have been clarified by recent molecular studies, but species-level relationships within this group have not been thoroughly addressed. In this study, we sampled specimens representing every recognized species of these genera, in addition to 2 outgroup taxa, and used target enrichment to sequence thousands of ultraconserved element (UCE) loci, as well as mitochondrial DNA reconstructed from off-target reads, from each specimen to infer these relationships. We constructed both concatenation and coalescent-based estimates of phylogeny from this dataset using matrices of varying levels of completeness, and we generated a time-scaled ultrametric tree using a recently published fossil-based external calibration. We found uniformly strong support for a monophyletic subfamily Euphoniinae and genus Chlorophonia, but a paraphyletic Euphonia across UCEs and mitochondrial genomes. Otherwise, our inferred relationships were largely concordant with previous studies. Our time-tree indicated a stem divergence time of 13.8 million years ago for this lineage, followed by a relatively young crown age of only 7.1 million years. Reconstructions of biogeographic history based on this tree suggest a South American origin for crown Euphoniinae, possibly resulting from a transoceanic dispersal event from the Eastern Hemisphere, followed by 2 dispersal events into the Caribbean and as many as 6 invasions of North America coinciding with recent estimates of the age at which the Isthmus of Panama had completely formed. We recommend splitting Euphonia and resurrecting the genus Cyanophonia for the 3 blue-hooded species more closely related to Chlorophonia. Based on our results, we suspect there is undescribed species-level diversity in at least one, possibly many, widespread and phenotypically-diverse species.

Methods

Full methods for DNA extraction, target capture, library preparation, Illumina sequencing, bioinformatics, and phylogenetic analyses are provided in our manuscript. Briefly, we extracted whole DNA from tissue and toe pad specimens housed in natural history collections and sent these DNAs to Arbor Biosciences for target capture of the Tetrapod 5K UCE set and library preparation, following their myBaits and myReads protocols. Sequencing was performed on an Illumina NextSeq 550 platform at the University of Minnesota Genomics Center. Bioinformatics processing of read data followed the PHYLUCE pipeline, and UCE loci and assembled mitogenomes were analyzed using concatenation and coalescent-based methods with both maximum likelihood and Bayesian frameworks.

Usage Notes

A metadata file has been included that describes the format and content of each file in our dataset.

Funding

Bell Museum of Natural History, University of Minnesota, Award: Richard & Judie Huempfner Avian Research Grant 2017

American Museum of Natural History, Award: Frank Chapman Memorial Fund Grant 2017

National Science Foundation, Award: DEB-1541312