There are four datasets used for constructing the phylogenetic trees in "A systematic study of North American Angelica species (Apiaceae) based on nrDNA ITS and cpDNA sequences and fruit morphology" Dataset I included the entire ITS region from 231 representatives of Apiaceae subfamily Apioideae. Datasets II-IV each included the same 176 accessions and comprised all 26 NA species of Angelica (represented by 132 accessions) and other related species. Dataset II included the entire ITS region; Dataset III included the cpDNA ndhF-rpl32, rpl32-trnL, and psbM-psbD regions; and Dataset IV included combined ITS and cpDNA data.

DNA sequences were initially aligned using Muscle (www.ebi.ac.uk/Tools/msa/muscle) and its default pairwise and multiple alignment parameters, then rechecked and adjusted manually as necessary using MEGA7. Gaps were positioned to minimize nucleotide mismatches. Uncorrected pairwise nucleotide differences were determined using PAUP* vers. 4.0b10

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