The opportunistic pathogen Pseudomonas aeruginosa uses quorum sensing to control the expression of multiple virulence factors. In this dataset, we provide raw data demonstrating that periodically disturbing biofilms composed of P. aeruginosa using a physical force reduces the expression of multiple quorum sensing regulated virulence factors from the three major regulons. This dataset also contains cell density measurements of bacteria both the in biofilm and planktonic state under a variety of conditions that use physical force to manipulate the spatial positioning of bacteria. Finally, the data set contains raw outputs from a network logic model that described the core quorum sensing network. This data is affiliated with the manuscript entitled "Periodically disturbing biofilms reduces expression of quorum sensing regulated virulence factors in Pseudomonas aeruginosa."