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Data from: RADseq resolves the Hawaiian Island radiation of Myrsine L. (Primulaceae) and provides evidence for hybridization

Citation

Appelhans, Marc; Paetzold, Claudia; Wood, Kenneth; Wagner, Warren (2020), Data from: RADseq resolves the Hawaiian Island radiation of Myrsine L. (Primulaceae) and provides evidence for hybridization, Dryad, Dataset, https://doi.org/10.5061/dryad.6wwpzgmvs

Abstract

The Hawaiian radiation of Myrsine (primrose family, Primulaceae) is the only one of the ten most species Hawaiian plant lineages that has never been included in a phylogenetic analysis. Our study is based on a RADseq dataset of nearly all Hawaiian Myrsine species and a Sanger sequencing dataset based on a worldwide sampling of Myrsine and related genera. Myrsine as a whole might be paraphyletic with respect to the monotypic Macaronesian genera Heberdenia and Pleiomeris, while Hawaiian Myrsine is resolved as monophyletic. Hawaiian Myrsine consists of three main lineages of which one contains the majority of species and is mainly confined to Kauaʻi, while the other two lineages primarily consist of few widespread species. While phylogenetic reconstructions delivered fully resolved and supported tree topologies, Quartet Sampling and HyDe analyses reveal phylogenetic incongruence throughout the phylogeny and provide the first molecular evidence of extensive hybridization in the lineage.

Methods

The dataset consists of Sanger (ITS, ETS) sequences and RADseq data. For the Sanger datasets, alignments are provided. The RADseq data has been processed with ipyrad 0.7.28 and de-multiplexed raw reads have been deposited at SRA (https://www.ncbi.nlm.nih.gov/sra; BioProject number PRJNA614459).

For the RADseq dataset, phylogenetic trees are provided, which have been calculated using ExaBayes 1.5 and RAxML 8.2.4. We also tested for phylogenetic incongruence and signs of hybridization in our datasets using Quartet Sampling v.1.2.1 and HyDe v.0.4.3. Phylogenetic trees with Quartet Sampling results and tables with the HyDe results are included here.