Data from: UDP-glycosyltransferases act as key determinants of host plant range in generalist and specialist Spodoptera species
Data files
Apr 17, 2024 version files 120.59 GB
Abstract
Phytophagous insects have evolved sophisticated detoxification systems to overcome the anti-herbivore chemical defenses produced by many plants. However, how these biotransformation systems differ in generalist and specialist insect species and their role in determining insect host plant range remains an open question. Here we show that UDP-glycosyltransferases (UGTs) play a key role in determining the host range of insect species within the Spodoptera genus. Comparative genomic analyses of Spodoptera species that differ in host plant breadth identified a relatively conserved number of UGT genes in generalist species but high levels of UGT gene pseudogenization in the specialist Spodoptera picta. CRISPR-Cas9 knockouts of the three main UGT gene clusters of Spodoptera frugiperda revealed that UGT33 genes play an important role in allowing this species to utilize the poaceous plants maize, wheat and rice, while UGT40 genes facilitate utilization of cotton. Further functional analyses in vivo and in vitro identified the UGT SfUGT33F32 as the key mechanism that allows generalist S. frugiperda to detoxify the benzoxazinoid DIMBOA, a potent insecticidal phytotoxin produced by poaceous plants. However, while this detoxification capacity is conserved in several generalist Spodoptera species, Spodoptera picta, which specializes on Crinum plants, is unable to detoxify DIMBOA due to a nonfunctionalizing mutation in SpUGT33F34. Collectively, these findings provide new insight into the role of insect UGTs in host plant adaptation, the mechanistic basis of evolutionary transitions between generalism and specialism and offer new molecular targets for controlling a group of notorious insect pests.
README: Data from: UDP-glucosyltransferases act as key determinants of host plant range in generalist and specialist Spodoptera species
https://doi.org/10.5061/dryad.6wwpzgn5w
- The transcriptomic raw data for different tissues of Spodoptera frugiperda: Head, Epidermis, Hemolymph, Fat body, Foregut, Midgut and Hindgut. Seven tissues from six instar larvae of the Spodoptera frugiperda XZ strain were dissected and prepared for transcriptome sequencing. Ten individuals were pooled as a biological replicate and three biological replicates were performed for each tissue. Extraction, quality and quantity analysis of total RNA, library preparation and sequencing were completed on the DNBSEQ gene sequencing platform (Huada Genomics Co., Ltd, Shenzhen, China).
- The assembled genome and annotation of Spodoptera picta: (1) spicta_final.fa, the assembled genome of Spodoptera picta. (2) spicta_final.gff3, the genome annotation of Spodoptera picta.
- The alignments of Spodoptera frugiperda UGT proteins with Fringe as outgroup, UGT33 and UGT40 from five Spodoptera species: (1) The alignments of Spodoptera frugiperda UGT proteins with Fringe as outgroup; (2) The alignments of UGT33 proteins from five Spodoptera species. (3) The alignments of UGT40 proteins from five Spodoptera species
- Sequence chromatograms for the CRISPR mutants: (1) Multiple peaks in chromatograms of directly sequenced PCR products of the G0 larvae indicate the presence of indel mutations in SeUGT33F24; (2) Multiple peaks in chromatograms of directly sequenced PCR products of the G0 larvae indicate the presence of indel mutations in SfUGT33F32; (3) Representative chromatogram derived from direct sequencing of PCR products flanking the sgRNA site of SeUGT33F24 from the WH-S-33F24 strain; (4) Representative chromatogram of direct sequencing of PCR products flanking the sgRNA site of SfUGT33F32 from the XZ-33F32 strain; (5) Representative chromatograms of direct sequencing of PCR products of individuals from XZ-d33c1 strain; (6) Representative chromatograms of direct sequencing of PCR products of individuals from XZ-d33c2 strain; (7) Representative chromatograms of direct sequencing of PCR products of individuals from XZ-d40c strain; (8) Representative sequencing chromatogram indicates only the wild type sequence present at the potential off-target site of the SeUGT33F7 gene in the WH-S-33F24 strain.
Methods
Usage notes: (1) The transcriptomic raw data for different tissues of Spodoptera frugiperda; (2) The assembled genome and annotation of Spodoptera picta; (3) The alignments of Spodoptera frugiperda UGT proteins with Fringe as outgroup, UGT33 and UGT40 from five Spodoptera species; (4) Sequence chromatograms for the CRISPR mutants