Data from: Larval diet and temperature alter mosquito immunity and development: using body size and developmental traits to track carry-over effects on longevity
Data files
Dec 01, 2023 version files 28.97 KB
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Mackay_etal_2023_code_and_data.zip
24.87 KB
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README.md
4.10 KB
Abstract
Background: Estimating arbovirus transmission potential requires a mechanistic understanding of how environmental factors influence the expression of adult mosquito traits. While preimaginal exposure to environmental factors can have profound effects on adult traits, tracking and predicting these effects remains challenging.
Methods: Using Aedes albopictus and a structural equation modelling approach, we explored how larval nutrition and temperature jointly affect development rate and success, body size, and whether these metrics capture carry-over effects on adult longevity. Additionally, we investigated how larval diet and temperature affect the baseline expression of ten immune genes.
Results: We found that larval development success was primarily determined by diet, while temperature and diet both affected development rate, pupal wet weight, and wing length. Effects of diet on both morphometric measures and their relationships to adult longevity were asymmetrical, with wing length having a positive association and pupal weight a negative association. Larval diet indirectly affected adult longevity, and the time to pupation was negatively correlated with longevity. The expression of eight immune genes from Toll, JAK-STAT, and Imd pathways was enhanced in mosquitoes with higher nutrition.
Conclusions: Our results highlight deficiencies from using a single body size measure to capture carry-over effects on adult traits. Further studies of larval development rate under varying environmental conditions and its potential for tracking carry-over effects on vectorial capacity are warranted.
Mackay, A.J., J. Yan, C.H. Kim, A.M.G. Barreaux, C.M. Stone. Larval diet and temperature alter mosquito immunity and development: using body size and developmental traits to track carry-over effects on longevity. Parasites and Vectors
Mackay_etal_2023_data_code_files
This zip directory contains the data files and r codes required to reproduce analyses in the associated paper evaluating effects of larval diet and temperature on Aedes albopictus juvenile development and survival, and female body size (pupal wet weight, wing length),longevity and immune gene expression.
Authors
Andrew J. Mackay
University of Illinois at Urbana-Champaign, Prairie Research Institute, Illinois Natural History Survey
1902 Griffith Drive, Champaign, IL 61820
amackay@illinois.edu
Jiayue Yan
University of Illinois at Urbana-Champaign, Prairie Research Institute, Illinois Natural History Survey
1902 Griffith Drive, Champaign, IL 61820
jiayue@illinois.edu
Chang-Hyun Kim
University of Illinois at Urbana-Champaign, Prairie Research Institute, Illinois Natural History Survey
1902 Griffith Drive, Champaign, IL 61820
maraychk@illinois.edu
Antoine Barreaux
INTERTRYP (Univ. Montpellier, CIRAD, IRD)
Montpellier, France
University of Bristol, School of Biological Sciences
Bristol, UK
antoine.barreaux@cirad.fr
Chris M. Stone
University of Illinois at Urbana-Champaign, Prairie Research Institute, Illinois Natural History Survey
1902 Griffith Drive, Champaign, IL 61820
cstone@illinois.edu
File list (files in Mackay_etal_2023_data_code_files.zip)
Mackay_et_al_2023_juvenile_develop_time.csv
Mackay_et_al_2023_juvenile_surv.csv
Mackay_et_al_2023_adult_F_size_long.csv
Mackay_et_al_2023_IGE_8genes_H25_L25_reference.csv
Mackay_et_al_2023_IGE_Hop_H25_L25_reference.csv
Mackay_et_al_2023_IGE_PIA_H25_L25_reference.csv
README_Mackay_et_al_2023_juvenile_develop_time.txt
README_Mackay_et_al_2023_juvenile_surv.txt
README_Mackay_et_al_2023_adult_F_size_long.txt
README_Mackay_et_al_2023_immune_gene expression.txt
Mackay_etal_2023_bodysize.R
Mackay_etal_2023_development.R
Mackay_etal_2023_immune_gene_expression.R
Mackay_etal_2023_longevity.R
File descriptions
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Mackay_et_al_2023_juvenile_surv.csv* - contains observations on survival to adult eclosion.
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Mackay_et_al_2023_juvenile_develop_time.csv* - contains observations on the development time to pupal and adult life stages for all individuals that successfully completed development.
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Mackay_et_al_2023_adult_F_size_longevity.csv* - contains measurements of female pupal wet weight, juvenile development time, wing length and adult longevity.
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Mackay_et_al_2023_IGE_8genes_H25_L25_reference.csv* - contains normalized values of relative fold change in the baseline expression of 8 immune genes (Spaetzle 1A, Toll 1B, Rel 1A, Cactus, Defensin C, Cecropin D, Domeless, Caspar) in 5-day old adult females.
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Mackay_et_al_2023_IGE_Hop_H25_L25_reference.csv* - contains normalized values of relative fold change in the baseline expression of the Hopscotch gene in 5-day old adult females.
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Mackay_et_al_2023_IGE_PIA_H25_L25_reference.csv* - contains normalized values of relative fold change in the baseline expression of the PIAS 2 gene in 5-day old adult females.
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Mackay_etal_2023_bodysize.R* - R code for reproducing ANCOVA, mixed-effects models and figures of female pupal wet weight and wing length.
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Mackay_etal_2023_development.R* - R code for reproducing mixed-effects model of juvenile survival, survivor sex ratio, and generalized linear models figures of male and female development time.
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Mackay_etal_2023_immune_gene_expression.R* - R code for reproducing generalized linear models and figures of relative changes in baseline immune gene expression in 5-day old adult females.
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Mackay_etal_2023_longevity.R* - R code for reproducing Cox PH model and multigroup pSEM of adult female longevity.
R software and associated package dependencies (https://www.r-project.org/) are required to open and execute the R code files.