Data from: Simultaneous determination of diosmin and hesperidin in combined pharmaceutical preparation by synchronous fluorescence spectrofluorimetric method
Data files
Mar 21, 2024 version files 32.03 KB
Abstract
Diosmin (DSM) and hesperidin (HSP) binary mixture was analyzed simultaneously by developing a sensitive, rapid, and simple synchronous spectrofluorimetric method. In methanol at ∆λ of 100 nm, the relative synchronous fluorescence intensities (RSFI) of both medications were measured. It was shown that these intensities were influenced by distinct experimental factors. The optimization and detailed study of these parameters were conducted. For DSM and HSP, respectively, the plots of synchronous fluorescence intensity-concentration were found to be rectilinear across the ranges of 0.5-5.0 µg/mL and 0.2-3.0 µg/mL. For DSM and HSP, respectively, 0.107 µg/mL and 0.048 µg/mL were the detection limits and 0.323 and 0.144 µg/mL were the limits of quantification. The method described in this study was effectively employed to estimate the quantities of both drugs present in commercially available mixed tablets. The results produced using this method were then compared favorably to results obtained using a different method for comparison.
README: Data from: Simultaneous determination of diosmin and hesperidin in combined pharmaceutical preparation by synchronous fluorescence spectrofluorimetric method
[https://doi.org/10.5061/dryad.70rxwdc5w]
Description of the data and file structure
File List:
1.Calibration_data_for_analysis_of_DSM_by_Synchronous_Fluorescence_ Spectrofluorimetric_Method.xlsx
A linear calibration curve was obtained when the RSFI was plotted against the final drug concentrations of DSM (µg/mL).
2.Calibration_data_for_analysis_of_HSP_by_Synchronous_Fluorescence_ Spectrofluorimetric_Method.xlsx
A linear calibration curve was obtained when the RSFI was plotted against the final drug concentrations of HSP (µg/mL).
Abbreviations:
RSFI is the relative synchronous fluorescence intensity.
n is the number of samples.
r is the regression coefficient.
SD is the standard deviation.
RSD is the relative standard deviation.
DSM is the abbreviation of Diosmin drug.
HSP is the abbreviation of Hesperidin drug.
Sharing/Access information
1. Licenses/restrictions placed on the data: No License needed for this data.
2. Links to publications that cite or use the data:
3. Was the data derived from another source? No
4. Is this dataset included in our main manuscript? No
5. Recommended citation for this dataset: H. Askar, M.E. Fathy, M.M. Tolba, Fatma A. Aly, and Mohammed E.-S. Metwally (2024), Simultaneous Determination of Diosmin and Hesperidin in Combined Pharmaceutical Preparation by Synchronous Fluorescence Spectrofluorimetric Method.
Code/Software
Instrument- or software-specific information needed to interpret the data: Eclipse fluorescence spectrophotometer was used to record measurements and fluorescence spectra and Agilent Technologies provided the Xenon flash lamp for it. The slit width was 5 nm, and the high voltage mode was used (800 v). Smoothing factor 15 was used. At 280 nm and 390 nm for DSM and HSP, respectively, the SF spectra were estimated.
Methods
Description of Method used for collection/generation of data:
A series of 10 mL volumetric flasks were filled with aliquots of the DSM and HSP standard solutions covering the working concentration range shown in Table 1. Then, methanol was added to dilute them to the mark. By scanning both monochromators with 10 nm excitation and emission windows and a constant wavelength difference (Δλ) of 100 nm, SF spectra of the solutions were captured. For DSM and HSP, the SF spectra's intensities were calculated to be at 280 nm and 390 nm, respectively. The experiment was run in parallel with a blank one. To obtain the calibration curves, plotting of the RSFI with the final drug concentrations (µg/mL) was done. An alternative was to derive the corresponding regression equations.