Data from: A transgenic quail model that enables dynamic imaging of amniote embryogenesis
Data files
Aug 05, 2015 version files 9.05 GB
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HH11 mCherry Dapi Tiled Z Stack 20x.lsm.001
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HH11 mCherry Dapi Tiled Z Stack 20x.lsm.002
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HH11 mCherry Dapi Tiled Z Stack 20x.lsm.003
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HH11 mCherry Dapi Tiled Z Stack 20x.lsm.004
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HH11 mCherry Dapi Tiled Z Stack 20x.lsm.005
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HH11 mCherry Dapi Tiled Z Stack 20x.lsm.006
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HH11 mCherry Dapi Tiled Z Stack 20x.lsm.007
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HH5 mCherry Dapi 20x Tiled Z Stack.lsm.001
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HH5 mCherry Dapi 20x Tiled Z Stack.lsm.002
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HH5 mCherry Dapi 20x Tiled Z Stack.lsm.003
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HH5 mCherry Dapi 20x Tiled Z Stack.lsm.004
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HH5 mCherry Dapi 20x Tiled Z Stack.lsm.005
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HH5 mCherry Dapi 20x Tiled Z Stack.lsm.006
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HH5 mCherry Dapi 20x Tiled Z Stack.lsm.007
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README_for_HH11 mCherry Dapi Tiled Z Stack 20x.lsm.txt
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Stage X unincubated PGK H2B Cherry 20x 1x tiled Z stack 2 stiched.lsm.001
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Stage X unincubated PGK H2B Cherry 20x 1x tiled Z stack 2 stiched.lsm.002
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Stage X unincubated PGK H2B Cherry 20x 1x tiled Z stack 2 stiched.lsm.003
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Stage X unincubated PGK H2B Cherry 20x 1x tiled Z stack 2 stiched.lsm.004
Abstract
Embryogenesis is the coordinated assembly of tissues during morphogenesis through changes in individual cell behaviors and collective cell movements. Dynamic imaging, combined with quantitative analysis, is ideal for investigating fundamental questions in developmental biology involving cellular differentiation, growth control and morphogenesis. However, a reliable amniote model system that is amenable to the rigors of extended, high-resolution imaging and cell tracking has been lacking. To address this shortcoming, we produced a novel transgenic quail that ubiquitously expresses nuclear localized monomer cherry fluorescent protein (chFP). We characterize the expression pattern of chFP and provide concrete examples of how Tg(PGK1:H2B-chFP) quail can be used to dynamically image and analyze key morphogenetic events during embryonic stages X to 11.