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Rapid mechanical stimulation of inner-ear hair cells by photonic pressure

Citation

Abeytunge, Sanjeewa; Gianoli, Francesco; Hudspeth, A. James; Kozlov, Andrei S. (2021), Rapid mechanical stimulation of inner-ear hair cells by photonic pressure, Dryad, Dataset, https://doi.org/10.5061/dryad.76hdr7sww

Abstract

Hair cells, the receptors of the inner ear, detect sounds by transducing mechanical vibrations into electrical signals. From the top surface of each hair cell protrudes a mechanical antenna, the hair bundle, which the cell uses to detect and amplify auditory stimuli, thus sharpening frequency selectivity and providing a broad dynamic range. Current methods for mechanically stimulating hair bundles are too slow to encompass the frequency range of mammalian hearing and are plagued by inconsistencies. To overcome these challenges, we have developed a method to move individual hair bundles with photonic force. This technique uses an optical fiber whose tip is tapered to a diameter of a few micrometers and endowed with a ball lens to minimize divergence of the light beam. Here we describe the fabrication, characterization, and application of this optical system and demonstrate the rapid application of photonic force to vestibular and cochlear hair cells.

Methods

Data were collected as described in the manuscript.

Usage Notes

Each dataset corresponds to a different figure. The data are saved in .txt files where the columns are time, laser redout and photodiode measurements. The traces reported in the manuscript are averages over 25 repetitions and the uploaded files contain every trace. The .log files contain all other relevant information: stimuls type, duration, delay, intensity of the laser, outputs, range of frequencies, et cetera. The file "Data_Fig5_Histogram.txt" includes the Time, X position, Y position and Vector X+Y of the hair bundle oscillations before and after laser irradiation used to compose the histogram in Figure 5. 

Funding

Howard Hughes Medical Institute

Wellcome Trust, Award: 108034/Z/15/Z