This dataset contains the supporting scripts and data used to analyze co-culture fluorescent colony images in high-throughput. It contains some example images that can be used in the scripts.

It also contains the script used to plot and determine the maximum growth rates of growth curves of some of the mutants and the script used to stitch overlapping microscope images.

Methods are included in the following manuscript (link below to biorxiv, in review at eLife): Three-dimensional biofilm growth supports a mutualism involving matrix and nutrient sharing manuscript by Arjes et al.

Usage notes are included on each script and accompanying ReadMe files when appropriate.