Skip to main content
Dryad logo

Data from: Dynamic Wolbachia prevalence in Acromyrmex leaf-cutting ants: potential for a nutritional symbiosis

Citation

Andersen, Sandra B.; Boye, Mette; Nash, David R.; Boomsma, Jacobus J. (2012), Data from: Dynamic Wolbachia prevalence in Acromyrmex leaf-cutting ants: potential for a nutritional symbiosis, Dryad, Dataset, https://doi.org/10.5061/dryad.7fq7d558

Abstract

Wolbachia are renowned as reproductive parasites, but their phenotypic effects in eusocial insects are not well understood. We used a combination of qrt-PCR, fluorescence in situ hybridisation, and laser scanning confocal microscopy to evaluate the dynamics of Wolbachia infections in the leaf-cutting ant Acromyrmex octospinosus across developmental stages of sterile workers. We confirm that workers are infected with one or two widespread wsp genotypes of Wolbachia, show that colony prevalences are always 100%, and characterize two rare recombinant genotypes. One dominant genotype is always present and most abundant while another only proliferates in adult workers of some colonies and is barely detectable in larvae and pupae. An explanation may be that Wolbachia genotypes compete for host resources in immature stages while adult tissues provide substantially more niche space. Tissue-specific prevalences of the two genotypes differ, with the rarer genotype being overrepresented in the adult foregut and thorax muscles. Both genotypes occur extra-cellularly in the foregut, suggesting an unknown mutualistic function in worker ant nutrition. Both genotypes are also abundant in the faecal fluid of the ants, suggesting that they may have extended functional phenotypes in the fungus garden that the ants manure with their own faeces.

Usage Notes