Various species of freshwater fish regulate the expression of certain proteins in response to environmental contamination. Previous research has shown that CYP1A expression increases in response to contaminant levels and can result in increased tumor formation. Fish in contaminated environments would thus benefit by downregulating the expression of CYP1A to reduce tumor prevalence as an adaptive strategy. Alternatively, monitoring of the CYP1A protein in fish can serve as a bioindicator of the pollution level of an environment. This study evaluated CYP1A expression in twelve different species of freshwater fish from seven bodies of water throughout western NY including Cuba Lake, Genesee River, Hanging Bog, Love Canal, Moss Lake, Rushford Lake and Tifft Nature Preserve. Western blot analysis was used to measure CYP1A expression as a marker of site pollution and potential fish population adaptation. It was hypothesized that low CYP1A expression at a site with known contamination would suggest signs of adaptation to pollution levels present. Furthermore, if at least one sample from a species showed CYP1A expression, then the CYP1A antibody (Caymen Chemical, USA; 173132) had compatibility with that species, eliminating falsely suspected adaptation. The results from this study suggest possible adaptation of fish may be occurring in the polluted Tifft Nature Preserve and Genesee River. --
Dataset collected: 12 freshwater fish species were collected from 7 sites in western New York, USA. using hoop nets
Dataset processing: fish were humanely sacrificed (MS-222), tissues were extracted and presevered in RNA later and then analyzed using western blot analysis. CYP1A protein expression was measured using the CYP1A antibody (Caymen Chemical, USA; 173132) and protein samples were normalized to actin (Millipore, USA; MAB1501) and a loading control with positive CYP1A expression (BR2).