Data for: Evidence to support phytosanitary policies - The minimum effective heat treatment parameters for pathogens associated with forest products
Data files
May 24, 2024 version files 69.08 KB
Abstract
Research on reducing the movement of pests on wood products has led to several options for safer trade including heat treatment of wood to mitigate pests. In this study, pathogenic organisms commonly regulated in the trade of forest products were tested to determine the minimum heat dose (temperature and time) required to cause mortality. The mycelial stage of tree pathogens, Heterobasidion occidentale, Grosmannia clavigera, Bretziella fagacearum, Phytophthora cinnamomi, P. lateralis, P. ramorum and P. xmultiformis, which may be found in wood products, were tested in vitro using the Humble water bath with parameters simulating the rate of heat applied to wood in a commercial kiln. The lethal temperature for the pathogens ranged from 44 to 50°C for a 30-minute treatment duration. A molecular diagnostic method to confirm pathogen mortality was available for five of the species heat treated. RNA detection using reverse transcription real-time PCR was used to validate pathogen mortality following treatment for: P. ramorum, P. lateralis, P. cinnamomi, P. xmultiformis and G. clavigera. The heat treatment data set is deposited here.
README: Data for: Evidence to support phytosanitary policies -The minimum effective heat treatment parameters for pathogens associated with forest products
https://doi.org/10.5061/dryad.7m0cfxq3k
Data are observations on post-treatment survival of Heterobasidion occidentale, Grosmannia clavigera, Bretziella fagacearum, Phytophthora cinnamomi, P. lateralis, P. ramorum and P. xmultiformis exposed to heat in a water bath at temperatures ranging from 40 up to 52 degrees Celsius for 30 minutes.
Description of the data and file structure
These data were assembled and summarized from raw data sheets and transferred into excel format.
Description of fields:
- Experiment number: The experimental run within which the data were collected
- Experiment date: The date on which the experimental run was conducted
- Species: The Latin name of the pathogen containing the first initial of the genus followed by the species name
- Isolate: The unique identifier for each specimen corresponding to the identity assigned by the holder of the culture collection from which the sample was received.
- Temperature_C: The temperature in degrees Celsius that each specimen was tested.
- Duration_Min: The treatment duration in minutes measured from when the last probe in the water bath reached the target treatment temperature
- Replicate: The replicate test of each isolate growing on independent grains, treated in the same run, under the same conditions.
- Survival: post treatment outcome, either Y or N, where Y is survival measured by observed mycelial growth and N is no growth observed indicating no survival. Missing data is indicated by a blank.
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Code/Software
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Methods
The data are observations on post-treatment survival of Heterobasidion occidentale, Grosmannia clavigera, Bretziella fagacearum, Phytophthora cinnamomi, P. lateralis, P. ramorum and P. xmultiformis exposed to heat in a water bath. The experiments were used to determine the optimal heating conditions for treatment of wood infested with forest pathogens. The mycelial stage of pathogens growing on grain were exposed to temperatures from 40 up to 52 degrees celsius for 30 minutes. Heat-treated and unexposed control grains with mycelium were plated on 20% V8 agar for Phytophthora species or 2% malt extract agar with yeast extract for fungi and observed for growth to determine if they survived treatment at each of the exposure temperatures.