https://doi.org/10.5061/dryad.7pvmcvf1p

RNA-seq data were obtained from a captive population of zebra finches at Queen Mary University of London. Individuals were in their first breeding season, having formed breeding pairs and produced fertile eggs. Samples were collected in accordance with national guidelines. The left gonad was dissected from five males and five females, homogenised and stored in RNA later until preparation. We used the Animal Tissue RNA Kit (Qiagen) to extract RNA. Dual-indexed, strand-specific RNASeq libraries were prepared at the NERC Environmental Omics Facility (NEOF) - Liverpool using the NEBNext polyA selection and Ultra II Directional RNA library preparation kits. RNA was sequenced on the Illumina NovaSeq using S4 chemistry, resulting in an average of 74 million 150bp paired-end reads per sample. 

Description of the data and file structure

Fastq Reads

For each sample fastq files are formatted as below:

x-${sample}_xxxxxx_L00x_Rx.fastq.gz

e.g.

for sample 3, there are reads:

1-3_201014_L001-R1.fastq.gz

1-3_201014_L001-R2.fastq.gz

1-3_201002_L002-R1.fastq.gz

1-3_201002_L002-R2.fastq.gz

Metadata and sequencing Information

File "Sample ID TGU raw untrimmed.xlsx" contains information on the sex, naming, md5 values and number of reads once trimmed

File "ID22333_Indexes used.xlsx" contains information on sequencing concentrations and adapters used per sample

Extraction Information

File "TGU individuals RNA extractions.xlsx" contains information on sample collection, death, extraction, sex, and extractions per sample

File "2100 expert_Eukaryote Total RNA Nano_DE24802221_2020-02-26_17-25-28.pdf" contains BioAnalyser output

File "Bio_analyser_README.txt" contains formation on naming for bioanalyser output