PLEASE NOTE: an additional file will be added to this data package: R script to calculate D and Gst for mtDNA (from a fasta file) and the associated p-values. It is also available here: . ABSTRACT: The spatial structure of host-parasite coevolution is shaped by population structure and genetic diversity of the interacting species. We analyzed these population genetic parameters in three related ant species: the parasitic slavemaking ant Protomognathus americanus and its two host species Temnothorax longispinosus and T. curvispinosus. We sampled throughout their range, genotyped ants on six to eight microsatellite loci and an MtDNA sequence and found high levels of genetic variation and strong population structure in all three species. Interestingly, the most abundant species and primary host, T. longispinosus, is characterized by less structure, but lower local genetic diversity. Generally, differences between the species were small, and we conclude that they have similar evolutionary potentials. The coevolutionary interaction between this social parasite and its hosts may therefore be less influenced by divergent evolutionary potentials, but rather by varying selection pressures. We employed different methods to quantify and compare genetic diversity and structure between species and genetic markers. We found that Jost D is well suited for these comparisons, as long as mutation rates between markers and species are similar. If this is not the case, for example, when using MtDNA and microsatellites to study sex-specific dispersal, model-based inference should be used instead of descriptive statistics (such as D or GST). Using coalescent-based methods, we indeed found that males disperse much more than females, but this sex bias in dispersal differed between species. The findings of the different approaches with regard to genetic diversity and structure were in good accordance with each other.
P.americanusGenepop
For each species there are three files, one with the geographic distances and one with the microsatellite data in Genepop format and one with the MtDNA sequences.
The species are: Protomagnathus americanus, Temnothorax curvispinosus, Temnothorax longispinosus.
In the genepop files, the first two letters of the name of the individual indicate where it is from. WV is West-Virginia, MA Massachusets, MD Maryland etc. OH is Ohio1 and O2 is Ohio2.
P.ameforGenepop.txt
T.curvispinosusGenepop
For each species there are three files, one with the geographic distances and one with the microsatellite data in Genepop format and one with the MtDNA sequences.
The species are:
Protomagnathus americanus,
Temnothorax curvispinosus,
Temnothorax longispinosus.
In the genepop files, the first two letters of the name of the individual indicate where it is from. WV is West-Virginia, MA Massachusets, MD Maryland etc. OH is Ohio1 and O2 is Ohio2.
T.curforGenepop.txt
T.longispinosusGenepop
For each species there are three files, one with the geographic distances and one with the microsatellite data in Genepop format and one with the MtDNA sequences.
The species are:
Protomagnathus americanus,
Temnothorax curvispinosus,
Temnothorax longispinosus.
In the genepop files, the first two letters of the name of the individual indicate where it is from. WV is West-Virginia, MA Massachusets, MD Maryland etc. OH is Ohio1 and O2 is Ohio2.
T.lonforGenepop.txt
T.curvispinosusGeogDistances
For each species there are three files, one with the geographic distances and one with the microsatellite data in Genepop format and one with the MtDNA sequences.
The species are:
Protomagnathus americanus,
Temnothorax curvispinosus,
Temnothorax longispinosus.
In the genepop files, the first two letters of the name of the individual indicate where it is from. WV is West-Virginia, MA Massachusets, MD Maryland etc. OH is Ohio1 and O2 is Ohio2.
T.curGeogDistances.csv
T.longispinosusGeogDistances
For each species there are three files, one with the geographic distances and one with the microsatellite data in Genepop format and one with the MtDNA sequences.
The species are:
Protomagnathus americanus,
Temnothorax curvispinosus,
Temnothorax longispinosus.
In the genepop files, the first two letters of the name of the individual indicate where it is from. WV is West-Virginia, MA Massachusets, MD Maryland etc. OH is Ohio1 and O2 is Ohio2.
T.longGeogDistances.csv
P.americanusGeogDistances
For each species there are three files, one with the geographic distances and one with the microsatellite data in Genepop format and one with the MtDNA sequences.
The species are:
Protomagnathus americanus,
Temnothorax curvispinosus,
Temnothorax longispinosus.
In the genepop files, the first two letters of the name of the individual indicate where it is from. WV is West-Virginia, MA Massachusets, MD Maryland etc. OH is Ohio1 and O2 is Ohio2.
P.ameGeogDistances.csv
T.curvispinosus36SeqsMtDNA
For each species there are three files, one with the geographic distances and one with the microsatellite data in Genepop format and one with the MtDNA sequences.
The species are:
Protomagnathus americanus,
Temnothorax curvispinosus,
Temnothorax longispinosus.
In the genepop files, the first two letters of the name of the individual indicate where it is from. WV is West-Virginia, MA Massachusets, MD Maryland etc. OH is Ohio1 and O2 is Ohio2.
Curvi_pops_36Seqs.fas
T.longispinosus50SeqsMtDNA
For each species there are three files, one with the geographic distances and one with the microsatellite data in Genepop format and one with the MtDNA sequences.
The species are:
Protomagnathus americanus,
Temnothorax curvispinosus,
Temnothorax longispinosus.
In the genepop files, the first two letters of the name of the individual indicate where it is from. WV is West-Virginia, MA Massachusets, MD Maryland etc. OH is Ohio1 and O2 is Ohio2.
Tlong50Seqs.fas
P.americanus_78seqsMtDNA
For each species there are three files, one with the geographic distances and one with the microsatellite data in Genepop format and one with the MtDNA sequences.
The species are:
Protomagnathus americanus,
Temnothorax curvispinosus,
Temnothorax longispinosus.
In the genepop files, the first two letters of the name of the individual indicate where it is from. WV is West-Virginia, MA Massachusets, MD Maryland etc. OH is Ohio1 and O2 is Ohio2.
Pam_78seqs.fas
RSamplingLocations
R script to plot the sampling locations
LocationsDec
Names and coordinates of the sampling locations
P.am_tandem
Same as Genepop file, but now in Tandem format
T.cur_tandem
Same as Genepop file, but now in Tandem format
T.longispinosus_tandem
Same as Genepop file, but now in Tandem format
T.lon_tandem.csv
RscriptMicroSatsWithPermutation
R script to calculate D and Gst and the associated p-values
RAllPops_D_Gst_MtDNA_PenningsJEB2011
This is R code to calculate Jost D and Gst and the associated p-values (using a permutation test) for MtDNA sequences. There are two versions. The first one (RPairw_D_MtDna_Penningsjeb2011.r) calculates pairwise values (between all pairs of populations in your data) and the second one (RAllPops_D_MtDNA_PenningsJEB2011.r) calculates one global value.
RPairw_D_Gst_MtDna_Penningsjeb2011
This is R code to calculate Jost D and Gst and the associated p-values (using a permutation test) for MtDNA sequences. There are two versions. The first one (RPairw_D_MtDna_Penningsjeb2011.r) calculates pairwise values (between all pairs of populations in your data) and the second one (RAllPops_D_MtDNA_PenningsJEB2011.r) calculates one global value.
RScript_MtDNA_JostD_and_Gst_Permutation