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Microsatellite data for Aedes aegypti populations in Florida and southern California

Citation

Pless, Evlyn; Powell, Jeffrey (2021), Microsatellite data for Aedes aegypti populations in Florida and southern California, Dryad, Dataset, https://doi.org/10.5061/dryad.83bk3j9p7

Abstract

In the affiliated paper we compare likely the oldest populations of Aedes aegypti in continental North America with some of the newest to illuminate the range of genetic diversity and structure that can be found within the invasive range of this important disease vector. Aedes aegypti populations in Florida have likely persisted since the 1600-1700s, while populations in southern California derive from new invasions that occurred in the last ten years. For this comparison, we genotyped 1,193 individuals from 29 sites at 12 highly variable microsatellites and a subset of these individuals at 23,961 single nucleotide polymorphisms (SNPs).

Methods

As in Brown et al. (2011), all individuals were genotyped at 12 highly variable microsatellite loci: four with trinucleotide repeats (A1, B2, B3, and A9) and eight with di-nucleotide repeats (AC2, CT2, AG2, AC4, AC1, AC5, AG1, and AG4). Microsatellite calls were made using GeneMapper. Any individuals that genotyped at fewer than 10 loci were excluded from analysis.

Usage Notes

The file is in STRUCTURE format, and missing data is represented with -9. See Table 1 in the manuscript or the other attachment to understand the population abbreviations and learn more about the sites.

Funding

National Institutes of Health, Award: RO1 AI101112