Understanding the fate of shrimp aquaculture effluent in a mangrove ecosystem: aiding management for coastal conservation
Cite this dataset
Hargan, Kathryn et al. (2020). Understanding the fate of shrimp aquaculture effluent in a mangrove ecosystem: aiding management for coastal conservation [Dataset]. Dryad. https://doi.org/10.5061/dryad.8sf7m0cj0
Abstract
Methods
Please see the paper Hargan et al. in Journal of Applied Ecology for a description of the collection methods and more details on sample preparation.
Dried bulk sediment subsamples from the top 1 cm of surface sediment were sieved (to remove shells, rootlets, and stones), ground, and ~30.0 mg was weighed and folded into 9x10 mm tin capsules for %N and δ15N. Acidified sediment samples (~12.0 - 20.0 mg) were weighed into separate 9 x 5 mm tin capsules for %C and δ13C analysis. Approximately 5.0 mg of ground mangrove and seagrass leave sample was utilized for stable isotope analysis. The 13C:12C and 15N:14N ratios were analyzed by continuous-flow isotope-ratio mass spectrometry. The spectrometer (ThemoFisher Delta V Plus stable-isotope analyzer coupled with a Flash Elemental Analyzer) was operated in dual isotope mode at The David W. and Claire B. Oxtoby Environmental Isotope Lab (Pomona College, California).
Analytical precision was determined using Acetanilide for δ13C (–29.53‰) and δ15N (1.18‰) (Indiana University) and %C (41.86%) and %N (16.18%) (ThermoFisher Scientific). For both δ13C and δ15N, the standard deviation of acetanilide on 10 replicate samples was < 0.2‰. Samples were calibrated to the standards USGS40 and USGS24 for 13C, and USGS40 and IAEA N-2 for 15N. Results of stable isotope analyses are reported in δ notation where δ = [(Rsample/Rstandard) – 1] x 1000. Rsample are the ratios of the isotopes (i.e. 13C/12C, 15N/14N) in samples, and Rstandard are the ratios of isotopes in Vienna Pee Dee Belemnite for δ13C and atmospheric nitrogen for δ15N.
Funding
National Geographic Society, Award: NGS-370R-18