Supplementary information provided with Murray et al.: Discovery of an Antarctic ascidian-associated uncultivated Verrucomicrobia with antimelanoma palmerolide biosynthetic potential
Murray, Alison E et al. (2021), Supplementary information provided with Murray et al.: Discovery of an Antarctic ascidian-associated uncultivated Verrucomicrobia with antimelanoma palmerolide biosynthetic potential, Dryad, Dataset, https://doi.org/10.5061/dryad.8sf7m0cpp
See the publication for details (main text and supplemental methods section). Briefly, we used a metagenomic approach to sequence a microbial community from an Antarctic ascidian, Synoicum adareanum. From this we assembled a nearly complete metagenome assembled genome (MAG) that encodes a candidate palmerolide A biosynthetic gene cluster of a verrucomicrobium affiliated new bacterium named in this manuscript as Candidatus Synoicihabitans palmerolidicus.
The supplemental material provided in this data set includes (i) Figure S1 which provides correlation coefficients derived between real time PCR data (methods described in the manuscript) and amplicon sequence variant (ASV) occurrences across a data set of 63 samples. (ii) Figure S2 displays the taxonomic classification of predicted proteins in the assembled MAG derived from an annotation pipeline called MetaERG. (iii) The annotation of the Ca. S. palmerolidicus genome, as a GFF file formatted file.
The GFF File needs to be read in a genome editing program.
National Institutes of Health, Award: CA205932
National Science Foundation, Award: OPP-0442857
National Science Foundation, Award: ANT-0838776
National Science Foundation, Award: PLR-1341339