https://doi.org/10.5061/dryad.8w9ghx3vp

Data for "Anthocyanin impacts multiple plant-insect interactions in a carnivorous plant."

Describes data on arthropod prey capture, herbivory, specialist insect larvae (inquiline) recruitment, seed set, and germination of anthocyanin-producing (red) and anthocyanin-free (green) morphs of the pitcher plant Sarracenia purpurea. Also includes data on LCMS characterization of these anthocyanin compounds, UV-vis spectrometer measurements from each color morph, data on change in color morph ratios over time, and a longitudinal study of prey capture of red morph plants. Change in color morph ratio data includes historical data from Case, Frederick W. "Some Michigan records for Sarracenia purpurea forma heterophylla." Rhodora 58.692 (1956): 203-207.

Description of the data and file structure

Files

Raw data files:

FAHSprey.csv -- Abundance of each prey morphospecies

LongitudinalStudy.csv -- weekly insect counts of different prey (order level) at a monomorphic (red morph) bog

MorphRatios.csv -- Historical and recent data on morph ratios at three polymorphic bogs

seedGerm.csv -- the germination rate from each individual that produced seed

SpurLC-MS_MS.csv -- mass spec response values for cyanidin-3-glucoside and cyanidin for young and old leaves of red and green morphs

MorphSpectra.csv -- spectral reflectance data from green and red morphs (both red and green regions of the leaf)

Cleaned data files:

CombinedDataset.csv -- Combined, pre-cleaned field and experimental data on herbivory, prey capture, and inquiline abundance

Glossary:

CombinedDataset.csv

pairID -- unique pair ID for one red and one green morph growing in close proximity

morph -- morph, either red (anthocyanin producing) or green (anthocyanin free)

perforation -- 0 = leaf intact below water line; 1 = leaf punctured below water line (does not include tears)

height -- height of experimental leaf, in cm

seedCount -- the number of intact seeds produced by the plant, NA indicates no attempt to flower; 0 indicates unsuccessful flowering attempt or no intact / viable seed

seedMass -- the total combined mass of all intact seeds, in g; NA indicates no attempt to flower; 0 indicates unsuccessful flowering attempt or no intact / viable seed

flowering -- 1 if flowering, 0 if not flowering; attempts to flower (e.g. aborted but, bud eaten) were scored as 1

insituProp -- the proportion of leaf area removed by (wild) herbivores from 1 leaf per plant in situ in the bog; NA indicates leaf too senesced to measure herbivory

insitumm2 -- the area of leaf removed (mm2) by (wild) herbivores from 1 leaf per plant in situ in the bog; NA indicates scan was not made when leaf was fresh (proportion herbivory is a post hoc estimate)

cupIDp -- the cup number (1-5) where a caterpillar was reared for the performance (P) experiment, NA = leaf too senesced for herbivory bioassays

areaEatenP -- the area consumed by a caterpillar from a 2 cm2 square of tissue during the performance experiment, NA = leaf too senesced for herbivory bioassays

propEatenP -- the proportion of the leaf consumed by a caterpillar from a 2 cm2 square of tissue during the performance experiment, NA = leaf too senesced for herbivory bioassays

preweightP -- the initial weight (in g) of each caterpillar used in the performance experiment, NA = leaf too senesced for herbivory bioassays

postweightP -- the final weight (in g) of each caterpillar used in the performance experiment, NA = leaf too senesced for herbivory bioassays

rgr -- relative growth rate of each caterpillar; (log(postweight) - log(preweight))/1.5 #experiment was run for 1.5 days, NA = leaf too senesced for herbivory bioassays

preweightC -- the initial weight (in g) of each caterpillar used in the choice experiment, NA = leaf too senesced for herbivory bioassays

cupIDc -- the cup number (1-5) where a caterpillar was reared for the choice (C) experiment, NA = leaf too senesced for herbivory bioassays

propEatenC -- the proportion of the leaf consumed by a caterpillar from a 2 cm2 square of tissue during the choice experiment, NA = leaf too senesced for herbivory bioassays

areaEatenC -- the area consumed by a caterpillar from a 2 cm2 square of tissue during the choice experiment, NA = leaf too senesced for herbivory bioassays

preyCount -- the number of individual prey items in each pitcher, NA indicates pitcher contained no fluid and could not captured prey or host inquilines

preyMass -- the mass (g) of prey insects (whole and in pieces) collected from each pitcher, NA indicates pitcher contained no fluid and could not captured prey or host inquilines

wymTotal -- the number of Wyeomyia smithii larvae in each pitcher, NA indicates pitcher contained no fluid and could not captured prey or host inquilines

met -- the number of Metriocnemius knabi larvae in each pitcher, NA indicates pitcher contained no fluid and could not captured prey or host inquilines

sarc -- the number of Fletcherimyia fletcheri larvae in each pitcher, NA indicates pitcher contained no fluid and could not captured prey or host inquilines

FAHSprey.csv

pairID -- unique pair ID for one red and one green morph growing in close proximity

morph -- morph, either red (anthocyanin producing) or green (anthocyanin free)

tube -- 1 == tube of prey contents was created at time of collection; 0 = no tube (no prey present when leaf collected)

mass -- the mass (g) of prey contents; "missing" refers to lines where a tube was recorded as collected, but could not be located for weighing,

NP = tube collected but contained no prey, and NA the pitcher contained no fluid and could not capture prey

beemass -- for those pitchers that captured a Bombus impatiens bumblebee, the total dry mass of all bees captured, in g

unknown:mite -- morpho species or undifferentiated members of an order (e.g. "hym" is any Hymenopteran not in a morphospecies category); empty and NA cells refer to 0 counts

of that taxon for that pitcher, and are corrected to zeros on lines 65 to 75 of Anthocyanin_loss.RMD (subsection "Rarefied diversity calculation" in knitted RMD)

met, wym, wym_pupa, sarc -- counts of inquiline larvae (see CombinedDataset for species names), empty and NA cells refer to 0 counts of that taxon for that pitcher, and

are corrected to zeros on lines 65 to 75 of Anthocyanin_loss.RMD (subsection "Rarefied diversity calculation" in knitted RMD)

eggs_binary -- 1 == small round eggs present in sample; 0 == eggs absent

LongitudinalStudy.csv

plantID -- unique plant ID for each individual plant (collected prey from 1 leaf per plant). All plants were red morphs (anthocyanin-producing)

date -- sample collection date month/day/year

springtail:snail -- counts of morphospecies, families, or undifferentiated members of an order ("Wmite" = water mite)

frass -- counts of frass (separate chunks) in leaf

leaf:seed -- counts of plant material; some plant material is subdivided into more specific categories

Metriocnemius:Sarcophagid -- counts of each of the three insect inquiline species; "Sarcophagid" refers to Fletcherimyia fletcheri in paper

spideParasite -- 1 indicates spider parasitizing pitcher (building web across opening)

algage -- 1 indicates the presence of algage

unknown -- counts of unknown animal mass

no_fluid -- 1 indicates pitcher was empty of fluid, making prey capture impossible

damage -- 1 indicates pitcher was damaged, preventing fluid acumulation

mL -- number of mL of water accumulated in control tubes of water (50mL falcon tubes) left in the bog

pitcherTotal -- the total number of pitchers per plant; entered only for September 1st

senescing -- 1 indicates pitcher is beginning to senece on that day

putrified -- 1 indicates pitcher fluid is anoxic, smells of decay, and/or is opaque or viscous with biofilm; 0 indicates clear, scentless fluid, no putrefaction

notes -- notes, especially on prey ID

MorphRatios.csv

year -- year of data collection; 1954 data from Case 1955 and 2022 data are presented in this paper

year_col -- "year color", placeholder column for graphing

bog -- a numeric assesion for each bog assigned by Case 1955

plot -- ID number for each 9x9 foot plot (per Case 1955); two plots per bog

red -- number of red plants (includes seedlings and mature plants) in each plot

green -- number of green morph (anthocyanin-free) plants (includes seedlings and mature plants) in each plot

density -- the total number of plants in each standardized area plot; sum of red and green columns

ratio -- the ratio between red and green counts in each plot; red/green columns

seedGerm.csv

plantID -- combined pairID and morph (g = green, r = red) for each unique plant that bore seed

potID -- integer identification for each pot with seed from a particular mother (e.g. 16r pot 1, 16r pot 2, etc); 1:n_pots

seeds -- number of seeds planted in a given pot

seedlings -- number of seedlings germinated in given pot

sum -- sum of the gr_seedling, rd_seedling, and dead columns (see below); redundant with "seedlings" column; created as a double check

gr_seedlings -- number of green morph seedlings

rd_seedlings -- number of red morph seedlings

dead -- number of seedlings that germinated and then died before scoring of seedling morph

tray -- integer identification for each tray containing 10 pots

date -- planting date month/day/year

morph -- long-form morph of mother

rate -- germination rate (seedlings column divided by seeds column)

parent -- long-form pairID of mother

SpurLC-MS_MS.csv

morph -- morph, either red (anthocyanin producing) or green (anthocyanin free)

age -- leaf age, either young (tender, pale green) or mature ("old", fully expanded and lignified)

sample -- sample number; replicates within the same leaf

C3G_MS -- the mass spec intensity for cyanidin-3-glucoside

Cy_MS -- the mass spec intensity for cyanidin

MorphSpectra.csv

wl -- wavelength of reflectance (nm)

All other column names take the form

[tissue name] [plant ID] [leaf a,b, or c] [replicate]

ex: grSpot 001 a .1

Tissues:

bodyRed -- the main 'vase' part of a red morph leaf

rdSpot -- a red area on the hood of a red morph

grSpot -- a green area on the hood of a green morph

hood -- any part of the hood of a green morph

plantIDs:

001, 002, and 003 -- red morphs

004, 005, and 006 -- green morphs

leaf:

a,b,c -- the first, second, and third leaf measured on a plant, respectively

replicate:

" ", ".1", ".2" -- the first, second, and third measurement taken for single area of leaf

Sharing/Access information

Historical color morph ratio data from Case, Frederick W. "Some Michigan records for Sarracenia purpurea forma heterophylla." Rhodora 58.692 (1956): 203-207.

Code/Software

Required R packages:

tidyverse, glmmTMB, performance, DHARMa, vegan, pavo

('see' and 'patchwork' are dependencies)

Code:

Anthocyanin_loss.Rmd -- Code for data analysis and making plots from pre-cleaned, combined morph field and experimental data

Anthocyanin_loss.html -- Knitted version of Anthocyanin_loss.Rmd file

VisualModels.Rmd -- Code for cleaning spectra data, maping onto pre-built pavo visual models, and plotting in vision model colorspace

VisualModels.html -- Knitted version of Visual Models RMD