Data from: Molecular profiling of diatom assemblages in tropical lake sediments using taxon-specific PCR and Denaturing High-Performance Liquid Chromatography (PCR-DHPLC)
Epp, Laura S, University of Potsdam
Stoof-Leichsenring, Kathleen R, University of Potsdam
Trauth, Martin H, University of Potsdam
Tiedemann, Ralph, University of Potsdam
Published Mar 30, 2011 on Dryad.
Cite this dataset
Epp, Laura S; Stoof-Leichsenring, Kathleen R; Trauth, Martin H; Tiedemann, Ralph (2011). Data from: Molecular profiling of diatom assemblages in tropical lake sediments using taxon-specific PCR and Denaturing High-Performance Liquid Chromatography (PCR-DHPLC) [Dataset]. Dryad. https://doi.org/10.5061/dryad.9011
Here we present a protocol to genetically detect diatoms in sediments of the Kenyan tropical Lake Naivasha, based on taxon-specific PCR amplification of short fragments (approx. 100 bp) of the small subunit ribosomal (SSU) gene and subsequent separation of species-specific PCR products by PCR-based DHPLC. An evaluation of amplicons differing in primer specificity to diatoms and length of the fragments amplified demonstrated that the number of different diatom sequence types detected after cloning of the PCR products critically depended on the specificity of the primers to diatoms and the length of the amplified fragments whereby shorter fragments yielded more species of diatoms. The DHPLC was able to discriminate between very short amplicons based on the sequence difference, even if the fragments were of identical length. and if the amplicons differed only in a small number of nucleotides. Generally the method identified the dominant sequence types from mixed amplifications. A comparison with microscopic analysis of the sediment samples revealed that the sequence types identified in the molecular assessment corresponded well with the most dominant species. In summary, the PCR-based DHPLC protocol offers a fast, reliable, and cost-efficient possibility to study DNA from sediments and other environmental samples with unknown organismic content, even for very short DNA fragments.
This table includes results from cloning and direct sequencing after DHPLC collection, along with the Genbank accession numbers of the retrieved sequences. Reference is made to Figure 3 in the paper.