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Separable gain control of ongoing and evoked activity in the visual cortex by serotonergic input

Citation

Azimi, Zohre et al. (2020), Separable gain control of ongoing and evoked activity in the visual cortex by serotonergic input, Dryad, Dataset, https://doi.org/10.5061/dryad.931zcrjgk

Abstract

Controlling gain of cortical activity is essential to modulate weights between internal ongoing communication and external sensory drive. Here, we show that serotonergic input has separable suppressive effects on the gain of ongoing and evoked visual activity. We combined optogenetic stimulation of the dorsal raphe nucleus (DRN) with wide-field calcium imaging, extracellular recordings, and iontophoresis of serotonin (5-HT) receptor antagonists in the mouse primary visual cortex. 5-HT1A receptors promote divisive suppression of spontaneous activity, while 5-HT2A receptors act divisively on visual response gain and account for normalization of population responses over a range of visual contrasts in awake and anesthetized states. Thus, 5-HT input provides balanced but distinct suppressive effects on ongoing and evoked activity components across neuronal populations. Imbalanced 5-HT1A/2A activation, either through receptorspecific drug intake, genetically predisposed irregular 5-HT receptor density, or change in sensory bombardment may enhance internal broadcasts and reduce sensory drive and vice versa.

Methods

Data are recorded from a series of optogenetic experiments combined with wide-field calcium imaging and multi-unit electrophysiology from the visual cortex of ePetCre mice in the anesthetized and the awake state. Also, in two sets of experiments, 5-HT1A and 5-HT2A receptors were blocked in vivo via iontophoretic application of these receptors antibody in the visual cortex. Traces of all the data that used to produce the figures in the manuscript are provided under the name of that figure. For details of the experimental paradigm please read the method section of the manuscript.

Usage Notes

Traces of all the data that used to produce the figures in the manuscript are provided under the name of that figure. For details of the experimental paradigm please read the method section of the manuscript.

Funding

Deutsche Forschungsgemeinschaft (DFG), Award: Dirk Jancke JA 945/4-1

Deutsche Forschungsgemeinschaft (DFG), Award: Dirk Jancke JA 945/5-1

Deutsche Forschungsgemeinschaft (DFG), Award: Stefan Herlitze, Dirk Jancke SFB 874, project number 122679504

Deutsche Forschungsgemeinschaft (DFG), Award: Dirk Jancke JA 945/3-1, SL 185/1-1

Deutsche Forschungsgemeinschaft (DFG), Award: Stefan Herlitze HE 2471/12-1

Deutsche Forschungsgemeinschaft (DFG), Award: Stefan Herlitze 2471/18-1

Deutsche Forschungsgemeinschaft (DFG), Award: Melanie D Mark MA 5806/1-2

Deutsche Forschungsgemeinschaft (DFG), Award: Melanie D Mark MA 5806/2-1