Glucose transporter expression and regulation following a fast in the ruby-throated hummingbird, Archilochus colubris
Ali, Raafay Syed et al. (2020), Glucose transporter expression and regulation following a fast in the ruby-throated hummingbird, Archilochus colubris, Dryad, Dataset, https://doi.org/10.5061/dryad.98sf7m0h4
Hummingbirds, subsisting almost exclusively on nectar sugar, face extreme challenges to blood sugar regulation. The capacity for transmembrane sugar transport is mediated by the activity of facilitative glucose transporters (GLUTs) and their localisation to the plasma membrane (PM). In this study, we determined the relative protein abundance of GLUT1, GLUT2, GLUT3, and GLUT5 via immunoblot using custom antibodies in whole-tissue and PM fractions of flight-muscle, heart, and liver of ruby-throated hummingbirds (
This dataset is the raw normalised arbitrary intensity of Western blots of GLUT1, GLUT2, GLUT3, and GLUT5 from either whole-tissue homogenates ("ffGLUTdata_cell.csv") or plasma-membrane fractions ("ffGLUTdata_pm.csv") of the flight muscle, heart, and liver of fed or fasted ruby-throated hummingbirds. Each antibody band has been normalised to the corresponding total-protein stain (Sypro Ruby Red) using BioRad ImageLab software. "BirdID" refers to the internal numbering scheme for each unique hummingbird. "Blot" refers to unique immunoblot membranes.
RScripts that were used process this data into mixed-effects models, estimate the model means, and plot the output are also provided ("GLUT ANALYSIS v2.R")
Data are also provided for the analysis of circulating sugars and metabolite concentrations with the R script entitled "ff2018 Metabolomics.R".
Please make sure to have all R libraries loaded in the order they are presented in. Please note the the R scripts are not self-executable and are designed for use with R-Studio.
Natural Sciences and Engineering Research Council of Canada, Award: 386466
Human Frontier Science Program, Award: RGP0062/2016