Zbtb14 regulates monocyte and macrophage development through inhibiting pu.1 expression in zebrafish
Deng, Yun et al. (2022), Zbtb14 regulates monocyte and macrophage development through inhibiting pu.1 expression in zebrafish, Dryad, Dataset, https://doi.org/10.5061/dryad.9cnp5hqms
To elucidate the mechanism underlying the aberrant monocyte/macrophage development of zbtb14-/- mutant, GFP-positive cells were isolated from either wild-type Tg(mpeg1.1:eGFP) or zbtb14 -/- //Tg(mpeg1.1:eGFP) larvae at 2dpf by FACS. mRNA was extracted from sorted cells using RNeasy Micro (Qiagen, Manchester, UK) and mRNA libraries were constructed using NEBNext Ultra RNA Library Prep Kit for52 Illumina and sequenced under Illumina HiSeq X Ten with pair end 150bp (PE150).
National Natural Science Foundation of China, Award: 32171097