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Data from: "Identification and assessment of single nucleotide polymorphisms (SNPs) between Culex complex mosquitoes." in Genomic Resources Notes Accepted 1 August 2014-30 September 2014

Citation

Kang, David S.; Sim, Cheolho (2014), Data from: "Identification and assessment of single nucleotide polymorphisms (SNPs) between Culex complex mosquitoes." in Genomic Resources Notes Accepted 1 August 2014-30 September 2014, Dryad, Dataset, https://doi.org/10.5061/dryad.9fg6h

Abstract

Culex pipiens complex mosquitoes are important vectors for many human pathogens including West Nile encephalitis, Rift Valley fever and Lymphatic filariasis. In this study we characterized a set of SNP markers between two biotypes of the Culex pipiens complex, Culex pipiens form molestus and Culex pipiens form pipiens, for use in a high-resolution genetic mapping and population genetics. DNA pooled from 10 specimens of each biotype were sequenced and analyzed for variation in 28 genes. The total of 4714 bp across orthologs of C. pipiens form pipiens and C. pipiens form molestus revealed 44 SNPs in the 3279 bp coding regions, 48 SNPs in the 1435 bp non-coding regions, and 10 indels. The ratio of transitions to transversions approached 2:1, with transitions constituting the majority of synonymous coding substitutions. The informative SNP markers were successfully identified and assessed from both C. pipiens biotypes. We expect that novel SNPs characterized in this study would be useful for genetic studies to elucidate the genetic basis of diverged eco-physiological traits between the two biotypes of the C. pipiens complex.

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