Skip to main content
Dryad

Data from: Phylogenomic loci define the generic boundaries of Gochnatieae and improve resolution at the species level in Moquiniastrum (Compositae)

Cite this dataset

Gostel, Morgan R. et al. (2024). Data from: Phylogenomic loci define the generic boundaries of Gochnatieae and improve resolution at the species level in Moquiniastrum (Compositae) [Dataset]. Dryad. https://doi.org/10.5061/dryad.9ghx3ffj6

Abstract

Understanding the evolution of the tribe Gochnatieae (Compositae) has been the subject of considerable effort in the past decade. This is due to the key position of this tribe in the phylogeny of the sunflower family and the corresponding implications for biogeographic and morphological evolution of Compositae. Previous studies have confirmed the monophyly of this tribe as well as most of the genera that belong to it. However, phylogenetic resolution of Gochnatieae at both the genus- and species-level has remained poor. A subset of new phylogenomic loci used in this study has proven effective and has improved phylogenetic resolution in this group. The results of this work demonstrate Gochnatieae is a well-supported clade comprised of nine genera (Anastraphia, Cnicothamnus, Cyclolepis, Gochnatia, Moquiniastrum, Nahuatlea,  Pentaphorus, Richterago, Tehuasca). One recently described genus, Vickia, was not included in this study; but its placement in Gochnatieae as a tenth genus in the tribe is well-justified. The monospecific Cyclolepis, which had been circumscribed within the tribe since its inception but was subsequently removed and designated as incertae sedis since 2014, is also shown to belong to Gochnatieae. We confirmed the monophyletic Moquiniastrum with two well-supported subclades. Ancestral area reconstruction analyses show that Gochnatieae originated in Eastern South America about 53 my. Apparently, except for Cyclolepis and Richterago, the ancestors of the other genera of Gochnatieae originated about 44 my from an area that now corresponds to the central Andes. The presence of the genera in the Chaco phytogeographic province, central Chile, and Mexico-United States-Caribbean is a result of dispersal from the central Andes. The ancestral distribution of Moquiniastrum corresponds to a large area comprising Eastern South America and the current central Andes, about 32 my. Ancestral character state reconstruction that included four characters indicates several states associated with complex plant reproductive biology such as gynodioecy, gynomonoecy, and polygamodioecy are derived in Gochnatieae as are heterogamous capitula (in Moquiniastrum and Richterago), dimorphic and subdimorphic corollas (in Cnicothamnus, Moquiniastrum, and Richterago), and the presence of marginal female corollas (in Moquiniastrum and Richterago). Within Moquiniastrum, two subclades (Densicephalum and Polymorphum) exhibit divergent patterns of trait evolution associated with these reproductive characters which suggests this genus can serve as a model to understand the sexual system evolution in plants.

README: Data from: Phylogenomic loci define the generic boundaries of Gochnatieae and improve resolution at the species level in Moquiniastrum (Compositae)

https://doi.org/10.5061/dryad.9ghx3ffj6

Description of the data and file structure

Char_Final3.csv corresponds to the parameter file used for ancestral character state reconstruction analyses, carried out using the phytools package in R. 

Dist_Areas_2022.csv corresponds to the parameter file used for ancestral area analyses carried out using BioGeoBEARS. Distribution of taxa analyzed in this study follows the areas defined as A-F and described in the manuscript. 

42_Concat_14June2021.xml corresponds to the parameter file used for divergence dating analyses carried out in this study using the software, BEAST. 

Each .fasta file corresponds to multiple sequence alignments for each molecular marker analyzed in this study. The name of the .fasta file corresponds to the name of each locus analyzed, following the "Working Locus Identifier" described in Appendix 2 of the manuscript. 

Sharing/Access information

n/a

Methods

High-throughput sequencing libraries were generated using microfluidic PCR-based target enrichment and sequenced on an Illumina MiSeq. Sequences were demultiplexed, cleaned, assembled, and then aligned per locus. Geographic areas were selected based on occurrence data gathered from herbarium records for all taxa included in this study. Ancestral character state matrices were constructed from herbarium collections and taxonomic knowledge by the taxonomic specialists who are authors of this manuscript.

Funding

Smithsonian Institution

Consejo Nacional de Investigaciones Científicas y Técnicas, Award: BID-2017-0965

National Council for Scientific and Technological Development, Award: 307272/2019-2