Data from: "Transcriptome sequence identity between Lyme disease tick vectors, Ixodes scapularis and Ixodes ricinus" in Genomic Resources Notes accepted 1 April 2014 to 31 May 2014
Contreras, Marinela, University of Castile-La Mancha
Tobes, Raquel, Oh no sequences! Research group; Era7 Bioinformatics, Plaza Campo Verde no. 3 Ático 18001 Granada Spain
Grubhoffer, Libor, University of South Bohemia in České Budějovice, Institute of Parasitology
Estrada-Peña, Agustin, University of Zaragoza
de la Fuente, Jose, Oklahoma State University
Published Jul 29, 2014 on Dryad.
Cite this dataset
Contreras, Marinela et al. (2014). Data from: "Transcriptome sequence identity between Lyme disease tick vectors, Ixodes scapularis and Ixodes ricinus" in Genomic Resources Notes accepted 1 April 2014 to 31 May 2014 [Dataset]. Dryad. https://doi.org/10.5061/dryad.9js92
Ixodes scapularis and I. ricinus transmit the Lyme disease agent Borrelia burgdorferi in the U.S. and Europe, respectively. The only tick genome sequence available is that of I. scapularis, which constitutes a limitation for tick research. Recent evidences suggest that I. ricinus and I. scapularis transcriptomes share some degree of sequence identity. However, only the global transcriptome comparison reported here demonstrated that I. ricinus and I. scapularis share a 99.232±0.005 percent sequence identity with a very low frequency of INDELs. However, due to limitations of the current I. scapularis genome assembly, the number of aligned reads was only 26-27%. These results support the use of I. scapularis genome sequence as a reference for the analysis of I. ricinus transcriptomics and proteomics data, but addressing the limitations associated with the I. scapularis genome assembly.
BAI file corresponding to the BAM file of the Larvae samples Tophat accepted hits.
BAI file corresponding to the BAM file with the Glands samples Tophat accepted hits
FASTQ file with reads from Larvae samples
FASTQ file with the reads from Glands samples
BAM file containing the Tophat accepted hits of the Glands samples
BAM file containing the Tophat accepted hits of the Larvae samples
Commands run to map the reads to the reference genome, glads sample
Commands used to map the reads to the reference genome, Larvae sample