Data from: BrAD-seq: Breath Adapter Directional sequencing: a streamlined, ultra-simple and fast library preparation protocol for strand specific mRNA library construction
Townsley, Brad T., University of California, Davis
Covington, Michael F., University of California, Davis
Ichihashi, Yasunori, University of California, Davis
Zumstein, Kristina, University of California, Davis
Sinha, Neelima R., University of California, Davis
Published May 15, 2015 on Dryad.
Cite this dataset
Townsley, Brad T. et al. (2015). Data from: BrAD-seq: Breath Adapter Directional sequencing: a streamlined, ultra-simple and fast library preparation protocol for strand specific mRNA library construction [Dataset]. Dryad. https://doi.org/10.5061/dryad.9mq14
Next Generation Sequencing (NGS) is driving rapid advancement in biological understanding and RNA-sequencing (RNA-seq) has become an indispensable tool for biology and medicine. There is a growing need for access to these technologies although preparation of NGS libraries remains a bottleneck to wider adoption. Here we report a novel method for the production of strand specific RNA-seq libraries utilizing inherent properties of double-stranded cDNA to capture and incorporate a sequencing adapter. Breath Adapter Directional sequencing (BrAD-seq) reduces sample handling and requires far fewer enzymatic steps than most available methods to produce high quality strand-specific RNA-seq libraries. The method we present is optimized for 3-prime Digital Gene Expression (DGE) libraries and can easily extend to full transcript coverage shotgun (SHO) type strand-specific libraries and is modularized to accommodate a diversity of RNA and DNA input materials. BrAD-seq offers a highly streamlined and inexpensive option for RNA-seq libraries.
Compressed file containing Tomato RNA-seq reads from BrAD-seq shotgun (SHO) type strand-specific libraries (SHO_22.fastq, SHO_23.fastq, SHO_24.fastq).
Compressed file containing Tomato SAM RNA-seq reads from HTR-method libraries (S_HTR_A5.fastq, S_HTR_A6.fastq, S_HTR_B6.fastq, S_HTR_B7.fastq)